Characterization of Lancefield group C Streptococcus dysgalactiae isolated from farmed fish
Characterization of Lancefield group C Streptococcus dysgalactiae isolated from farmed fish
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DOI：
10.1111/j.1365-2761.2006.00763.x
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发表时间：
2006-11-01
影响因子：
2.5
通讯作者：
Yoshida, T.
中科院分区：
文献类型：
--
作者：
Nomoto, R.;Unose, N.;Yoshida, T.
由停乳链球菌引起的兰斯菲尔德C组链球菌（GCS）感染以尾柄的严重坏死病变为特征，已成为日本九州南部的养殖鱼如琥珀鱼、杜氏细尾鱼和黄尾鱼、五角细尾鱼的死亡率增加的原因。在这项研究中，酶谱的GCS菌株从鱼类和哺乳动物进行了调查，使用API ZYM系统，和GCS菌株的基因型特征进行了偏置正弦场凝胶电泳（BSFGE）。对鱼类和哺乳动物来源的GCS株16 S-23 SrDNA基因间隔区的部分序列进行了比较。API ZYM试验结果表明，分离的沙门氏菌很难区分。基于酶学变化的鱼类和动物的停乳。在BSFGE分析中，使用SmaI或ApaI作为限制性酶获得的宏观限制性图谱揭示了鱼类和动物分离株之间的差异。在16 S-23 SrDNA基因间隔区的部分序列中，所有鱼类分离物与所有哺乳动物分离物都有1 ~ 2个核苷酸的差异。探讨了S.鱼类分离株的BSFGE图谱也表明养殖鱼类中存在停乳菌株。
A Lancefield group C streptococcal (GCS) infection caused by Streptococcus dysgalactiae that is characterized by severe necrotic lesions of the caudal peduncle has been an increasing cause of mortality in farmed fish such as amberjack, Seriola dumerili, and yellowtail, Seriola quinqueradiata, in the southern part of Kyushu, Japan. In this study, enzymatic profiles of GCS strains from fish and mammals were investigated using the API ZYM system, and genotypic characterization of GCS strains was performed using biased sinusoidal field gel electrophoresis (BSFGE). The partial sequence of the 16S-23S rDNA intergenic spacer region of the GCS strain isolates from fish and mammals was also compared. The API ZYM test indicated that it is difficult to differentiate isolates of S. dysgalactiae from fish and animals based on enzymological variations. In the BSFGE analysis, the macrorestriction profiles, which were obtained using SmaI or ApaI as a restriction enzyme, revealed variations between the fish and animal isolates. The partial sequence of the 16S-23S rDNA intergenic spacer region of all the tested fish isolates differed from all mammalian isolates in one or two nucleotides. The possibility of a clonal expansion of S. dysgalactiae strains in farmed fish was also suggested by the BSFGE profiles of fish isolates.