Characterization of intervertebral disc degeneration by high-resolution magic angle spinning (HR-MAS) Spectroscopy
Characterization of intervertebral disc degeneration by high-resolution magic angle spinning (HR-MAS) Spectroscopy
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DOI：
10.1002/mrm.20392
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发表时间：
2005-03-01
影响因子：
3.3
通讯作者：
Kurhanewicz, J
中科院分区：
文献类型：
--
作者：
Keshari, KR;Zektzer, AS;Kurhanewicz, J
本研究的目的是确定高分辨率魔角旋转（HR-MAS）核磁共振波谱区分不同阶段的椎间盘退变（IVDD）的能力。从人尸体中取出17个椎间盘，并使用1D和2D（总相关光谱（TOCSY））1H HR-MAS光谱以及T和T-2弛豫时间测量对其进行分析，以确定椎间盘的化学组成和化学环境随变性水平增加的变化（Thompson等级）。其中重要的发现是，光谱是非常相似的样品从环形和核区域的光盘，和视觉上明显的变化，观察到光谱的环形和核样品从光盘与汤普森等级增加。N-乙酰胆碱（Cho）区域的面积比，和Cho碳水化合物（碳水化合物）区域的光谱允许我们区分光盘的增加汤普森等级与最小的重叠的个别比率。光盘光谱的化学成分的T1和T2弛豫时间的变化与变性程度没有显着相关性。本研究的结果支持使用体内光谱检测与椎间盘退变相关的化学变化的可行性。
The goal of this study was to determine the ability of high-resolution magic angle spinning (HR-MAS) NMR spectroscopy to distinguish different stages of intervertebral disc degeneration (IVDD). 17 discs were removed from human cadavers and analyzed them using 1 D and 2D (total correlation spectroscopy (TOCSY)) 1H HR-MAS spectroscopy, and T, and T-2 relaxation time measurements to determine the chemical composition and changes in chemical environment of discs with increasing levels of degeneration (Thompson grade). Among the significant findings were that spectra were very similar for samples taken from annular and nuclear regions of discs, and that visually apparent changes were observed in the spectra of the annular and nuclear samples from discs with increasing Thompson grade. Area ratios of the N-acetyl to choline (Cho) regions, and Cho to carbohydrate (Carb) regions of the spectra allowed us to discriminate between discs of increasing Thompson grade with minimal overlap of individual ratios. Changes in T, and T2 relaxation times of the chemical constituents of disc spectra were not significantly correlated to the degree of degeneration. The results of this study support the feasibility of using in vivo spectroscopy for detecting chemical changes associated with disc degeneration.