AGE-DEPENDENT ALTERATIONS IN RAT-LIVER MICROSOMAL NADPH-CYTOCHROME-C (P-450) REDUCTASE - A QUALITATIVE AND QUANTITATIVE-ANALYSIS
AGE-DEPENDENT ALTERATIONS IN RAT-LIVER MICROSOMAL NADPH-CYTOCHROME-C (P-450) REDUCTASE - A QUALITATIVE AND QUANTITATIVE-ANALYSIS
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DOI：
10.1016/0047-6374(83)90070-2
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发表时间：
1983-01-01
影响因子：
5.3
通讯作者：
WANG, RK
中科院分区：
文献类型：
--
作者：
SCHMUCKER, DL;WANG, RK
Hepatic drug-metabolizing capacities of rodents and man exhibit age-dependent declines. The extensive use of medication in geriatric patients demonstrates the need to characterize the mechanism(s) responsible for this reduced liver function. An analysis of microsomal NADPH-cytochrome c (P-450) reductase in young adult (3 mo.), mature (9 mo.) and senescent (27 mo.) male Fischer 344 rats revealed specific age-related qualitative and quantitative changes in this enzyme. Specific activity of the purified enzyme from young animals was 2-fold higher than that recovered from the other age groups. No change in molecular weight, alterations in heat inactivation profiles, an apparent reduction in substrate affinity and a 2-fold loss of enzyme activity per unit of immunoprecipitable material occurred as a function of animal age. Apparently, important liver drug-metabolizing enzyme undergoes post-translational modifications in its conformation which are reflected in the above parameters and which, ultimately, affect its efficacy.
