Exploring genome wide bisulfite sequencing for DNA methylation analysis in livestock: a technical assessment.
Exploring genome wide bisulfite sequencing for DNA methylation analysis in livestock: a technical assessment.
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DOI：
10.3389/fgene.2014.00126
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发表时间：
2014
影响因子：
3.7
通讯作者：
Couldrey C
中科院分区：
文献类型：
--
作者：
Doherty R;Couldrey C
在“组学”技术方面取得的最新进展正在推动牲畜选择和育种实践的革命。表观遗传机制，包括DNA甲基化，是控制哺乳动物基因表达的重要决定因素。DNA甲基化研究将有助于我们理解环境因素如何影响复杂生产和健康性状的表型变异。高通量测序是全面分析DNA甲基化的重要工具，基于亚硫酸氢盐的策略与DNA测序相结合，可以在基因组水平或基因组范围内进行定量的、特定部位的甲基化分析。简化代表性亚硫酸氢盐测序(RRBS)和最近的全基因组亚硫酸氢盐测序(WGBS)已被证明是研究人类和小鼠DNA甲基化的有效技术。在这里，我们报告了用于绵羊的RRBS和WGBS的开发，这是该技术在牲畜物种中的首次应用。研究和讨论了与这些方法相关的重要技术问题，包括片段大小选择和序列深度。
Recent advances made in “omics” technologies are contributing to a revolution in livestock selection and breeding practices. Epigenetic mechanisms, including DNA methylation are important determinants for the control of gene expression in mammals. DNA methylation research will help our understanding of how environmental factors contribute to phenotypic variation of complex production and health traits. High-throughput sequencing is a vital tool for the comprehensive analysis of DNA methylation, and bisulfite-based strategies coupled with DNA sequencing allows for quantitative, site-specific methylation analysis at the genome level or genome wide. Reduced representation bisulfite sequencing (RRBS) and more recently whole genome bisulfite sequencing (WGBS) have proven to be effective techniques for studying DNA methylation in both humans and mice. Here we report the development of RRBS and WGBS for use in sheep, the first application of this technology in livestock species. Important technical issues associated with these methodologies including fragment size selection and sequence depth are examined and discussed.
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影响因子：
7
作者：
Bai Y;Sartor M;Cavalcoli J
通讯作者：
Cavalcoli J
使用降低的表示硫酸盐测序来映射斑马鱼脑甲基甲基。
影响因子：
3.7
作者：
Chatterjee A;Ozaki Y;Stockwell PA;Horsfield JA;Morison IM;Nakagawa S
通讯作者：
Nakagawa S
影响因子：
46.9
作者：
通讯作者：
--
影响因子：
4.8
作者：
Smith, Zachary D.;Gu, Hongcang;Bock, Christoph;Gnirke, Andreas;Meissner, Alexander
通讯作者：
Meissner, Alexander
影响因子：
14.9
作者：
EHRLICH, M;GAMASOSA, MA;GEHRKE, C
通讯作者：
GEHRKE, C