Mutational Robustness of Ribosomal Protein Genes
Mutational Robustness of Ribosomal Protein Genes
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DOI：
10.1126/science.1194617
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发表时间：
2010-11-05
期刊：
影响因子：
56.9
通讯作者：
Andersson, Dan I.
中科院分区：
文献类型：
--
作者：
Lind, Peter A.;Berg, Otto G.;Andersson, Dan I.
突变的适应效应分布(DFE)对于了解进化动力学和复杂疾病以及保护受威胁物种具有重要意义。从DNA序列估计的DFE很少受到直接实验测试的影响。我们使用了一个细菌系统，在该系统中，以高灵敏度测量了两个核糖体蛋白中大量已定义的单一突变的适合性影响。获得的DFE似乎是单峰的，其中大多数突变(126个中的120个)是弱有害的，其余的可能是中性的。同义和非同义替换的DFE相似，这表明在一些基因中，信使RNA水平存在很强的适合性限制。
The distribution of fitness effects (DFE) of mutations is of fundamental importance for understanding evolutionary dynamics and complex diseases and for conserving threatened species. DFEs estimated from DNA sequences have rarely been subject to direct experimental tests. We used a bacterial system in which the fitness effects of a large number of defined single mutations in two ribosomal proteins were measured with high sensitivity. The obtained DFE appears to be unimodal, where most mutations (120 out of 126) are weakly deleterious and the remaining ones are potentially neutral. The DFEs for synonymous and nonsynonymous substitutions are similar, suggesting that in some genes, strong fitness constraints are present at the level of the messenger RNA.