MOLECULAR-CLONING OF THE HUMAN HISTAMINE H1 RECEPTOR GENE
MOLECULAR-CLONING OF THE HUMAN HISTAMINE H1 RECEPTOR GENE
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DOI：
10.1006/bbrc.1994.1786
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发表时间：
1994-06-15
影响因子：
3.1
通讯作者：
ITO, S
中科院分区：
文献类型：
--
作者：
FUKUI, H;FUJIMOTO, K;ITO, S
人组胺H1受体基因是用牛H1受体基因[Yamashita，M，Fukui，H，Sugama，K，Horio，Y.，Ito，S.，Mizuguchi，H和Wada，H(1991)proc.]克隆的。娜塔莉。阿卡德。SCI。美国88,11515-11519]用作探头。根据该基因的核苷酸序列推导出的受体蛋白由487个氨基酸残基组成，计算的相对分子质量为55781，具有GTP结合蛋白偶联受体的特征。在人类H1受体基因的5‘端序列中，我们定位了潜在的TATA盒和CACCC序列、AP1结合位点样序列、糖皮质激素反应元件样序列以及其他与诱导剂的结合序列。Northern印迹分析表明，H1受体mRNAs在胎盘、肺、骨骼肌和肾脏等外周组织中表达，显示为两条带，分别为3.0kb和3.5kb，而在脑中仅有一种表达。H1受体基因在胎盘中含量最丰富。用荧光原位杂交法将人H1受体基因定位于染色体3p25。(C)1994年学术出版社。
The human histamine H1 receptor gene, an intron-lacking gene, was isolated with bovine H1 receptor cDNA [Yamashita, M, Fukui, H, Sugama, K, Horio, Y., Ito, S., Mizuguchi, H, and Wada, H.(1991) Proc. Natl. Acad. Sci. USA 88, 11515-11519] used as a probe. The receptor protein deduced from the nucleotide sequence of this gene was composed of 487 amino acid residues with a calculated Mr of 55,781 and possessed characteristic properties of GTP binding protein-coupled receptors. At the 5' Ranking legion of the human H1 receptor gene, we located potential TATA box and CACCC sequences, AP1 binding site-like sequences, glucocorticoid responsive element-like sequences, and other binding sequences for inducers. Northern blot analysis showed that H1 receptor mRNAs visualized as two bands with 3.0-kilobase and 3.5-kilobase nucleotides were expressed in peripheral tissues such as placenta, lung, skeletal muscle, and kidney and that only one species with 3.5-kilobase nucleotides was present in the brain. H1 receptor mRNA was most abundant in the placenta. The human H1 receptor gene was mapped to the chromosome 3p25 by the fluorescence in situ hybridization method. (C) 1994 Academic Press, Inc.