Synthetic RNA-protein complex shaped like an equilateral triangle
Synthetic RNA-protein complex shaped like an equilateral triangle
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DOI：
10.1038/nnano.2010.268
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发表时间：
2011-02-01
影响因子：
38.3
通讯作者：
Saito, Hirohide
中科院分区：
文献类型：
--
作者：
Ohno, Hirohisa;Kobayashi, Tetsuhiro;Saito, Hirohide
利用自下而上的方法(1，2)构建了由核酸等生物大分子组成的合成纳米结构。特别是，Watson-Crick碱基配对已被用于构建各种二维和三维DNA纳米结构(3-10)。在这里，我们证明了RNA和核糖体蛋白L7Ae可以形成一个形状像等边三角形的纳米结构，它由三个蛋白质结合在一个RNA支架上组成。该复合体的构建依赖于与RNA(11-13)中扭折(K-Turn)基序结合的蛋白质，这使得RNA在三个位置可以弯曲类似60度，形成一个三角形。用这种方法构建的功能性RNA-蛋白质复合体可能在纳米医学(14，15)和合成生物学(14，16-18)中有应用。
Synthetic nanostructures consisting of biomacromolecules such as nucleic acids have been constructed using bottom-up approaches(1,2). In particular, Watson-Crick base pairing has been used to construct a variety of two-and three-dimensional DNA nanostructures(3-10). Here, we show that RNA and the ribosomal protein L7Ae can form a nanostructure shaped like an equilateral triangle that consists of three proteins bound to an RNA scaffold. The construction of the complex relies on the proteins binding to kink-turn (K-turn) motifs in the RNA(11-13), which allows the RNA to bend by similar to 60 degrees at three positions to form a triangle. Functional RNA-protein complexes constructed with this approach could have applications in nanomedicine(14,15) and synthetic biology(14,16-18).