Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
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DOI：
10.1016/j.celrep.2012.07.006
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发表时间：
2012-08-30
期刊：
影响因子：
8.8
通讯作者：
Olsen JV
中科院分区：
文献类型：
--
作者：
Lundby A;Lage K;Weinert BT;Bekker-Jensen DB;Secher A;Skovgaard T;Kelstrup CD;Dmytriyev A;Choudhary C;Lundby C;Olsen JV
Lysine acetylation is a major posttranslational modification involved in a broad array of physiological functions. Here, we provide an organ-wide map of lysine acetylation sites from 16 rat tissues analyzed by high-resolution tandem mass spectrometry. We quantify 15,474 modification sites on 4,541 proteins and provide the data set as a web-based database. We demonstrate that lysine acetylation displays site-specific sequence motifs that diverge between cellular compartments, with a significant fraction of nuclear sites conforming to the consensus motifs G-AcK and AcK-P. Our data set reveals that the subcellular acetylation distribution is tissue-type dependent and that acetylation targets tissue-specific pathways involved in fundamental physiological processes. We compare lysine acetylation patterns for rat as well as human skeletal muscle biopsies and demonstrate its general involvement in muscle contraction. Furthermore, we illustrate that acetylation of fructose-bisphosphate aldolase and glycerol-3-phosphate dehydrogenase serves as a cellular mechanism to switch off enzymatic activity.
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影响因子：
56.9
作者：
Choudhary, Chunaram;Kumar, Chanchal;Mann, Matthias
通讯作者：
Mann, Matthias
影响因子：
64.8
作者：
Malmström J;Beck M;Schmidt A;Lange V;Deutsch EW;Aebersold R
通讯作者：
Aebersold R
影响因子：
64.5
作者：
Huttlin EL;Jedrychowski MP;Elias JE;Goswami T;Rad R;Beausoleil SA;Villén J;Haas W;Sowa ME;Gygi SP
通讯作者：
Gygi SP
影响因子：
9.2
作者：
Bannister, AJ;Miska, EA;Kouzarides, T
通讯作者：
Kouzarides, T
影响因子：
5.3
作者：
Hoberg, JE;Popko, AE;Mayo, MW
通讯作者：
Mayo, MW