Augmentation of the human immune response by cyclophosphamide.
Augmentation of the human immune response by cyclophosphamide.
复制标题
环磷酰胺增强人体免疫反应。
作者：
D. Berd;M. Mastrangelo;P. Engstrom;A. Paul;H. Maguire
用环磷酰胺（CY）预处理实验动物，1 ~ 4天后可明显增强T细胞介导的对抗原的免疫力。我们研究了原发性迟发型超敏反应（DTH）和抗体的钥孔血蓝蛋白（KLH）和DTH的1-氯-2，4-二硝基苯（DNCB）在22例患者接受CY转移癌，12例黑色素瘤和10例结直肠癌。CY前16天，一半患者接受KLH，一半患者接受DNCB; CY后3天，接受KLH或DNCB，以最初未接受者为准。在抗原给药后14天抽血进行抗体滴度和/或皮肤试验。对于每种抗原，前CY患者的反应与后CY患者的反应进行了比较。我们发现，CY患者的预处理显著增加了DTH向KLH的发展。CY后3天给予KLH的患者组的DTH反应显著大于未给予CY的KLH患者组（中位数：单独KLH，0; CY后KLH，18 mm; p = 0.025）。与CY预处理相比，11例患者中有11例患者的DTH反应大于5 mm，而11例未使用CY的患者中有4例（p = 0.002）。当在没有CY的情况下给予DNCB时，没有患者发生DTH，而当在CY后3天给予DNCB时，11例患者中有3例发生DTH（p = 0.082）。CY预处理既不增强也不抑制对KLH的抗体应答;抗原后14天抗体阳性的患者比例为2/11例未接受CY预处理的患者和4/11例接受CY预处理的患者（p = 0.24）。CY预处理似乎导致原本无反应的患者出现DTH反应。对晚期癌症的T细胞无反应性的恢复可能导致对肿瘤相关抗原的免疫应答的增强。
Abstract Pretreatment of experimental animals with cyclophosphamide (CY) markedly potentiates the acquisition of T-cell-mediated immunity to an antigen given 1 to 4 days later. We have examined the primary induction of delayed-type hypersensitivity (DTH) and antibody to keyhole limpet hemocyanin (KLH) and DTH to 1-chloro-2,4-dinitrobenzene (DNCB) in 22 patients receiving CY for metastatic cancer, 12 with melanoma and 10 with colorectal carcinoma. Sixteen days before CY, one-half of the patients received KLH and one-half received DNCB; 3 days after CY, they received KLH or DNCB, whichever they had not received initially. Blood was drawn for antibody titer and/or skin testing was performed 14 days after administration of antigen. For each antigen, the responses of pre-CY patients were compared with those of post-CY patients. We found that pretreatment of patients with CY significantly augmented the development of DTH to KLH. The DTH reactions of the group of patients given KLH 3 days after CY were significantly greater than those of the group of patients given KLH without CY (medians: KLH alone, 0; KLH after CY, 18 mm; p = 0.025). With CY pretreatment, 11 of 11 patients developed a DTH response of greater than 5 mm compared with 4 of 11 patients without CY ( p = 0.002). No patient developed DTH to DNCB when it was given without CY whereas 3 of 11 patients developed DTH when DNCB was given 3 days after CY ( p = 0.082). CY pretreatment neither augmented nor suppressed the antibody response to KLH; the proportion of patients with antibody 14 days after antigen was 2 of 11 without CY and 4 of 11 with CY pretreatment ( p = 0.24). It appears that CY pretreatment resulted in the development of DTH responses in otherwise unreactive patients. Reversal of the T-cell anergy of advanced cancer could lead to augmentation of the immune response to tumor-associated antigens.
联合化疗对实体瘤患者外周血免疫调节细胞的影响：与反弹超调免疫功能恢复的相关性
DOI：
10.1016/0090-1229(81)90178-1
发表时间：
1981
期刊：
Clinical immunology and immunopathology
影响因子：
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作者：
Braun,DP;Harris,JE
通讯作者：
Harris,JE