Utilisation of molecularly imprinting technology for the detection of glucocorticoids for a point of care surface plasmon resonance (SPR) device
Utilisation of molecularly imprinting technology for the detection of glucocorticoids for a point of care surface plasmon resonance (SPR) device
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DOI：
10.1016/j.aca.2023.342004
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发表时间：
2023-11-16
影响因子：
6.2
通讯作者：
Turner，Nicholas W.
中科院分区：
文献类型：
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作者：
Blackburn，Chester;Sullivan，Mark V.;Turner，Nicholas W.
在这里，我们描述了四个合成的识别材料（nanoMIPs）选择性的糖皮质激素类固醇-泼尼松龙，泼尼松，地塞米松和可的松的合成和表征。使用固相合成方法，这些材料，然后应用于表面等离子体共振（SPR）传感器的发展，用于检测这四个目标在掺杂尿液，模仿农业废物的常规测试可能的环境暴露。合成的颗粒显示出104和160 nm之间的尺寸范围。进行了亲和力研究，并且这些合成材料显示出对其所需靶标的纳摩尔亲和力（15.9-62.8 nM）。此外，我们进行了交叉反应性研究，以评估材料对其所需目标的选择性，并且当与非所需目标相比时，材料显示出优异的选择性，并计算了选择性因子。此外，通过使用3D可视化，可以看出结构之间的微小变化（例如羟基到酮的转化）在化合物之间具有100倍以上的优异选择性。使用Surine™掺杂的样品，当与标准化缓冲液制备物相比时，材料对其靶标提供了相当的纳摩尔亲和力（10.7-75.7 nM）。尿液中所有化合物的检测水平均在纳摩尔范围内。所开发的传感器为这些设备提供了潜在的可能性，可用于防止这些药物化合物通过源头监测通过农业废物进入周围环境。同样，它们可用于监测临床样品中的使用。
Herein, we describe the synthesis and characterisation of four synthetic recognition materials (nanoMIPs) selective for the glucocorticoid steroids – prednisolone, prednisone, dexamethasone, and cortisone. Using a solid-phase synthesis approach, these materials were then applied in the development of a surface plasmon resonance (SPR) sensor for the detection of these four targets in doped urine, to mimic the routine testing of agricultural waste for possible environmental exposure. The synthesised particles displayed a range of sizes between 104 and 160 nm. Affinity studies were performed, and these synthetic materials were shown to display nanomolar affinities (15.9–62.8 nM) towards their desired targets. Furthermore, we conducted cross-reactivity studies to assess the materials selectivity towards their desired target and the materials showed excellent selectivity when compared to the non-desired target, with selectivity factors calculated. Furthermore, through the use of 3D visualisation it can be seen that small changes between structures (such as a hydroxyl to ketone transformation) there is excellent selectivity between the compounds in the ranges of 100 fold plus. Using Surine™ doped samples the materials offered comparable nanomolar affinities (10.7–75.7 nM) towards their targets when compared to the standardised buffer preparation. Detection levels in urine for all compounds was in the nanomolar range. The developed sensor offers potential for these devices to be used in the prevention of these pharmaceutical compounds to enter the surrounding environment through agricultural waste through monitoring at source. Likewise, they can be used to monitor use in clinical samples.