Migration of mesothelioma cells correlates with histotype-specific synthesis of extracellular matrix.
Migration of mesothelioma cells correlates with histotype-specific synthesis of extracellular matrix.
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间皮瘤细胞的迁移与细胞外基质的组织型特异性合成相关。
DOI：
10.3892/ijmm.4.1.67
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发表时间：
1999
影响因子：
5.4
通讯作者：
A. Modesti
中科院分区：
文献类型：
--
作者：
S. Scarpa;A. Giuffrida;M. Fazi;A. Coletti;C. Palumbo;H. Pass;A. Procopio;A. Modesti
本文研究了上皮样(E1)、纤维型(F1)和旁型(B1)三种人胸膜恶性间皮瘤细胞系(MM)合成细胞外基质(ECM)组分层粘连蛋白(Lm)、纤维连接蛋白(FN)和IV型胶原(Civ)，以及它们向ECM产生的蛋白的趋化和半趋化迁移。MM-B1合成和释放FN的能力最强，MM-E1产生的基底膜成分LM和Civ最多，MM-F1产生和释放Fn、LM和Civ的能力较弱。MM-B1具有最高的趋化和趋化活性，MM-F1向最低浓度的LM迁移，而对FN和Civ的趋化活性降低，MM-E1对各种ECM底物的迁移活性最低。我们证明了三种不同组织类型的MM具有不同的ECM产生的特征，这些差异决定了每个MM向ECM底物扩散和迁移的不同能力。
Three human pleural malignant mesothelioma cell cultures (MM) of epithelioid (E1), fibrous (F1) and byphasic (B1) histotype were studied in their synthesis of the extracellular matrix (ECM) components laminin (LM), fibronectin (FN), type IV collagen (cIV), and in their chemotactic and haptotactic migration towards the ECM produced proteins. MM-B1 showed the highest FN synthesis and release; MM-E1 produced the highest quantity of basement membrane constituents LM and cIV; MM-F1 weakly produced and released FN, LM and cIV. MM-B1 had the highest chemotactic and haptotactic motility, MM-F1 migrated toward the lowest concentration of LM while had reduced chemotactic activity toward FN and cIV; MM-E1 had the lowest migratory activity toward each ECM substrate. We demonstrated that three MM of different histotype are characterized by different ECM production and that these differences determine a variable ability of each MM to spread and migrate towards ECM substrates.