RESISTANCE OF MAIZE TO ANTHRACNOSE - CHANGES IN HOST PHENOLS AND PIGMENTS
RESISTANCE OF MAIZE TO ANTHRACNOSE - CHANGES IN HOST PHENOLS AND PIGMENTS
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DOI：
10.1094/phyto-67-251
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发表时间：
1977-01-01
期刊：
影响因子：
3.2
通讯作者：
NICHOLSON, RL
中科院分区：
文献类型：
--
作者：
HAMMERSCHMIDT, R;NICHOLSON, RL
研究了玉米（Zea mays）对禾生炭疽菌（Colletotrichum graminicola）的抗性，包括感病（Mo940）、抗病（H91）和抗过敏（33 - 16）自交系的酚代谢和病斑发展。从叶绿素含量和视觉观察，这是不可能区分敏感和超敏反应接种后42小时之前。然而，这些主机的反应，可以区分后62小时的超敏感的主机-病原体组合的叶绿素再生。宿主反应也可以通过苯酚含量的变化来区分。总酚含量（乙酸乙酯和水溶性酚）的所有3个自交系接种后随着时间的推移而增加。然而，酚含量在抗性和超敏抗性自交系增加48小时前检测到的敏感自交系的增加。在接种后42小时，乙酸乙酯可溶性酚类的抗性和超敏抗性自交系分别增加了约74%和110%，相比，在这些酚类的易感自交系中没有检测到增加。三种酚类化合物，M1，M2和M3，积累在每个自交系接种后。这些化合物在抗性和超敏反应-抗性相互作用中比在易感相互作用中积累得更早。化合物M1和M2经显色剂鉴定、薄层色谱分离和紫外光谱分析，确定为黄酮类黄酮化合物。M1和M2对C.禾生菌的孢子萌发和色谱生物测定。化合物M3与咖啡酸没有区别，对C.在测试的浓度下的禾生菌。花青苷色素在抗病和抗感寄主中积累，而在感病寄主中不积累。花色素苷积累后，在总酚含量的初始变化和增加后，在M1，M2和M3。酚类物质代谢在玉米抗病性中的作用。草生的建议，基于相关的病变限制与总酚化合物（化合物M1，M2和M3）的积累和花青素的外观。
The resistance of corn [Zea mays] to Colletotrichum graminicola was studied with respect to phenol metabolism and lesion development in susceptible (Mo940), resistant (H91), and hypersensitive-resistant (33-16) inbreds. From chlorophyll content and visual observations, it was impossible to distinguish susceptible and hypersensitive-resistant responses prior to 42 h after inoculation. These host responses could, however, be differentiated after 62 h by chlorophyll regeneration in the hypersensitive host-pathogen combination. Host responses could also be differentiated by changes in phenol content. Total phenol content (ethyl acetate- and water-soluble phenols) of all 3 inbreds increased with time after inoculation. However, phenol content in the resistant and hypersensitive-resistant inbreds increased 48 h prior to the increase detected in the susceptible inbred. At 42 h after inoculation the ethyl acetate-soluble phenols in the resistant and hypersensitive-resistant inbreds had increased by approximately 74 and 110%, respectively, as compared to no detectable increase in those phenols in the susceptible inbred. Three phenolic compounds, M1, M2 and M3, accumulated in each of the inbreds after inoculation. These compounds accumulated earlier in the resistant and hypersensitive-resistant interactions than in the susceptible interaction. Compounds M1 and M2 were suggested to be flavonoids of the flavone group based on tests with chromogenic reagents, separation by TLC, and UV spectral analysis. Both M1 and M2 were fungitoxic to C. graminicola in spore germination and chromatographic bioassays. Compound M3, which was not distinguishable from caffeic acid, was not inhibitory to C. graminicola at the concentrations tested. Anthocyanin pigments accumulated in the resistant and hypersensitive-resistant hosts, but not in the susceptible host. Anthocyanins accumulated after the initial changes in total phenol content and after the increase in M1, M2 and M3. A role for phenolic metabolism in resistance of corn to C. graminicola is suggested, based on a correlation of lesion restriction with the accumulation of total phenol compounds (compounds M1, M2 and M3) and the appearance of anthocyanins.