BED NUCLEUS OF THE STRIA TERMINALIS - CYTOARCHITECTURE, IMMUNOHISTOCHEMISTRY, AND PROJECTION TO THE PARABRACHIAL NUCLEUS IN THE RAT
BED NUCLEUS OF THE STRIA TERMINALIS - CYTOARCHITECTURE, IMMUNOHISTOCHEMISTRY, AND PROJECTION TO THE PARABRACHIAL NUCLEUS IN THE RAT
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DOI：
10.1002/cne.902830302
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发表时间：
1989-05-15
影响因子：
2.5
通讯作者：
GRAY, TS
中科院分区：
文献类型：
--
作者：
MOGA, MM;SAPER, CB;GRAY, TS
终纹床核（BST）向桥上的臂旁核（PB）发出密集的投影。BST包含许多不同类型的神经肽样免疫反应细胞和纤维，每一种都在细胞结构不同的BST亚核中表现出自己的特征分布。促肾上腺皮质激素释放因子(CRF)-、神经紧张素(NT)-、生长抑制素(SS)-和脑啡肽（ENK）样免疫反应（ir）神经元在BST投射到PB的区域内尤其多。在这项研究中，我们使用联合逆行荧光-免疫荧光方法来确定BST中投射到PB的神经元是否含有这些免疫反应。快速蓝注射到PB后，整个BST外侧部分有大量逆行标记的神经元，特别是在背外侧（DL）和后外侧亚核。逆行标记的神经元也存在于视前、腹侧外侧和包膜上的BST亚核和腹侧核。DL中许多CRF-ir、NT-ir和SS-ir神经元被逆行标记。在BST后外侧、腹外侧和囊上亚核中也发现了少量的双标记细胞，而ENK-ir神经元从未逆行标记。我们的研究结果表明，BST神经元投射到PB染色的神经肽与杏仁核中央核（CeA）中投射到PB的神经肽相同，进一步证明了这两种结构之间的密切解剖关系。
The bed nucleus of the stria terminalis (BST) sends a dense projection to the parabrachial nucleus (PB) in the pons. The BST contains many different types of neuropeptidelike immunoreactive cells and fibers, each of which exhibits its own characteristic distribution within cytoarchitecturally distinct BST subnuclei. Corticotropin releasing factor (CRF)-, neurotensin (NT)-, somatostatin (SS)-, and enkephalin (ENK)-like immunoreactive (ir) neurons are particularly numerous within areas of the BST that project to the PB. In this study, we use the combined retrograde fluorescence-immunofluorescence method to determine whether neurons in the BST that project to the PB contain these immunoreactivities. After Fast Blue injections into PB, retrogradely labeled neurons were numerous throughout the lateral part of the BST, particularly in the dorsal lateral (DL) and posterior lateral subnuclei. Retrogradely labeled neurons were also present in the preoptic, ventral lateral, and supracapsular BST subnuclei and in the parastrial nucleus. Many of the CRF-ir, NT-ir, and SS-ir neurons in DL were retrogradely labeled. A few double-labeled cells of each type were also found in the posterior lateral, ventral lateral and supracapsular BST subnuclei ENK-ir neurons were never retrogradely labeled. Our results show that BST neurons that project to the PB stain for the same neuropeptides as those in the central nucleus of the amygdala (CeA) that project to the PB, demonstrating further the close anatomical relations between these two structures.