Copper nanoparticles promote rapid wound healing in acute full thickness defect via acceleration of skin cell migration, proliferation, and neovascularization
Copper nanoparticles promote rapid wound healing in acute full thickness defect via acceleration of skin cell migration, proliferation, and neovascularization
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DOI：
10.1016/j.bbrc.2019.07.110
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发表时间：
2019-10-01
影响因子：
3.1
通讯作者：
Aghdami, Nasser
中科院分区：
文献类型：
--
作者：
Alizadeh, Sanaz;Seyedalipour, Bagher;Aghdami, Nasser
在世界范围内，受损的伤口愈合导致发病率和死亡率的巨大负担。目前的治疗有几个局限性。最近，纳米材料如铜纳米颗粒（CuNPs）吸引了相当大的研究兴趣。在这里，我们研究了各种CuNP浓度（1 μ M，10 μ M，100 μ M，1 mM和10 mM）和大小（20 nm，40 nm，80 nm）在伤口愈合中的潜在治疗效果。我们的研究结果表明，10 μ M浓度的40 nm的CuNPs和1 μ M浓度的80 nm的CuNPs是没有毒性的培养的成纤维细胞，内皮细胞和角质形成细胞，以及1 μ M浓度的80 nm的CuNPs增强内皮细胞迁移和增殖。对体内伤口愈合的广泛评估表明，1 μ M浓度的80 nm CuNP通过形成肉芽组织和更高的新血管在更短的时间内加速伤口愈合。重要的是，血清生化分析证实，40 nm CuNP（10 μ M）和80 nm CuNP（1 μ M）在伤口愈合期间在肝脏中没有显示任何积累。总的来说，我们的结果表明，1 μ M浓度的80 nm CuNP是一种有前途的NP，用于伤口愈合应用，没有不良副作用。(C)2019爱思唯尔公司All rights reserved.
Worldwide, impaired wound healing leads to a large burden of morbidity and mortality. Current treatments have several limitations. Recently, nanomaterials such as copper nanoparticles (CuNPs) have attracted considerable research interest. Here, we investigated the potential therapeutic effect of various CuNPs concentrations (1 mu M, 10 mu M, 100 mu M, 1 mM, and 10 mM) and sizes (20 nm, 40 nm, 80 nm) in wound healing. Our results revealed that the 10 mu M concentration of 40 nm CuNPs and the 1 mu M concentration of 80 nm CuNPs were not toxic to the cultured fibroblast, endothelial, and keratinocyte cells, and also 1 mu M concentration of 80 nm CuNPs enhanced endothelial cell migration and proliferation. Extensive assessment of in vivo wound healing demonstrated that the 1 mu M concentration of 80 nm CuNPs accelerated wound healing over a shorter time via formation of granulation tissue and higher new blood vessels. Importantly, serum biochemical analysis confirmed that the 40 nm CuNP (10 mu M) and 80 nm CuNP (1 mu M) did not show any accumulation in the liver during wound healing. Overall, our results have indicated that the 1 mu M concentration of 80 nm CuNPs is a promising NP for wound healing applications without adverse side effects. (C) 2019 Elsevier Inc. All rights reserved.