A guinea pig model of mesial temporal lobe epilepsy following nonconvulsive status epilepticus induced by unilateral intrahippocampal injection of kainic acid
A guinea pig model of mesial temporal lobe epilepsy following nonconvulsive status epilepticus induced by unilateral intrahippocampal injection of kainic acid
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DOI：
10.1111/j.1528-1167.2012.03669.x
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发表时间：
2012-11-01
期刊：
影响因子：
5.6
通讯作者：
de Curtis, Marco
中科院分区：
文献类型：
--
作者：
Carriero, Giovanni;Arcieri, Salvatore;de Curtis, Marco
目的：颞叶癫痫模型常用于研究局灶性癫痫的发生和发作。不同的实验室定义代表人类内侧颞叶的动物模型的标准可能不同。我们在此描述了一个局灶性癫痫模型的内侧颞（海马）起源，依赖于发作间期和发作脑电图（EEG）模式的分析，并与癫痫发作症状和神经病理学结果。该研究是基于豚鼠，一个物种很少用于开发慢性癫痫models.Methods：年轻的成年豚鼠双侧植入异氟烷麻醉下，硬膜外电极体感皮层和深度电极在CA 1海马区。将不锈钢引导套管单侧定位在右侧背侧海马中以注射1 μ l含有1 μ g红藻氨酸（KA）的0.9%NaCl溶液。术后1周，在注射KA前48 h和注射KA后每隔一周进行连续24 h/d视频EEG监测，
Purpose: Models of temporal lobe epilepsy are commonly utilized to study focal epileptogenesis and ictogenesis. The criteria that define animal models representative of human mesial temporal lobe may vary in different laboratories. We describe herein a focal epilepsy model of mesial temporal (hippocampal) origin that relies on the analysis of interictal and ictal electroencephalography (EEG) patterns and on their correlation with seizure symptoms and neuropathologic findings. The study is based on guinea pigs, a species seldom utilized to develop chronic epilepsy models.Methods: Young adult guinea pigs were bilaterally implanted under isoflurane anesthesia with epidural electrodes over somatosensory cortex and depth electrodes in CA1 hippocampal region. A stainless steel guide cannula was positioned unilaterally in the right dorsal hippocampus to inject 1 mu l of 0.9% NaCl solution containing 1 mu g kainic acid (KA). One week after surgery, continuous 24 h/day video-EEG monitoring was performed 48 h before and every other week after KA injection, for no