Optical projection tomography as a tool for 3D microscopy and gene expression studies
Optical projection tomography as a tool for 3D microscopy and gene expression studies
复制标题
DOI：
10.1126/science.1068206
复制
发表时间：
2002-04-19
期刊：
影响因子：
56.9
通讯作者：
Davidson, D
中科院分区：
文献类型：
--
作者：
Sharpe, J;Ahlgren, U;Davidson, D
三维（3D）光学显微镜的当前技术（去卷积，共聚焦。显微镜和光学相干断层扫描）通过“光学切片”样本来生成3D数据。这对可以成像的样品的最大厚度施加了严格的限制。我们已经开发出一种显微镜技术，使用光学投影断层扫描（OPT），以产生荧光和非荧光生物标本的厚度高达15毫米的高分辨率3D图像。OPT显微镜可以快速绘制完整胚胎或器官系统中RNA和蛋白质表达的组织分布，因此可以在发育生物学或基因功能的研究中发挥作用。
Current techniques for three-dimensional (3D) optical microscopy (deconvolution, confocal. microscopy, and optical coherence tomography) generate 3D data by "optically sectioning" the specimen. This places severe constraints on the maximum thickness of a specimen that can be imaged. We have developed a microscopy technique that uses optical projection tomography (OPT) to produce high-resolution 3D images of both fluorescent and nonfluorescent biological specimens with a thickness of up to 15 millimeters. OPT microscopy allows the rapid mapping of the tissue distribution of RNA and protein expression in intact embryos or organ systems and can therefore be instrumental in studies of developmental biology or gene function.