Identification of genes present specifically in a virulent strain of Klebsiella pneumoniae
Identification of genes present specifically in a virulent strain of Klebsiella pneumoniae
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DOI：
10.1128/iai.68.12.7149-7151.2000
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发表时间：
2000-12-01
影响因子：
3.1
通讯作者：
Chang, HY
中科院分区：
文献类型：
--
作者：
Lai, YC;Yang, SL;Chang, HY
肺炎克雷伯菌是败血症和尿路感染的常见原因。采用PCR支持的基因组消减杂交技术，对克雷伯氏菌强毒株中的特异性基因进行了鉴定。肺炎。对25个消减DNA克隆的分析揭示了19个不同的核苷酸序列。发现其中两个序列是编码Tn3926转座酶和胶囊多糖输出酶的基因。三个序列显示中等同源性与bvgAS，编码一个双组分的信号转导系统在百日咳博德特氏菌。其余序列与任何已知基因均不具有同源性。这些新序列在K. pneumoniae临床分离株，反映了K. pneumoniae人群
Klebsiella pneumoniae is a common cause of septicemia and urinary tract infections. The PCR-supported genomic subtractive hybridization was employed to identify genes specifically present in a virulent strain of K. pneumoniae. Analysis of 25 subtracted DNA clones has revealed 19 distinct nucleotide sequences. Two of the sequences were found to be the genes encoding the transposase of Tn3926 and a capsule polysaccharide exporting enzyme. Three sequences displayed moderate homology with bvgAS, which encodes a two-component signal transduction system in Bordetella pertussis. The rest of the sequences did not exhibit homology with any known genes. The distribution of these novel sequences varied greatly in K. pneumoniae clinical isolates, reflecting the heterogeneous nature of the K. pneumoniae population.