Silencing of hydroxycinnamoy-coenzyme A shikimate/quinate hydroxycinnamoyltransferase affects phenylpropanoid biosynthesis
Silencing of hydroxycinnamoy-coenzyme A shikimate/quinate hydroxycinnamoyltransferase affects phenylpropanoid biosynthesis
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DOI：
10.1105/tpc.020297
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发表时间：
2004-06-01
期刊：
影响因子：
11.6
通讯作者：
Legrand, M
中科院分区：
文献类型：
--
作者：
Hoffmann, L;Besseau, S;Legrand, M
在香豆酸莽草酸酯/奎尼酸酯的水平上引入苯丙素类化合物的3-位中的羟基，其合成涉及酰基转移酶活性。针对重组烟草（烟草）酰基转移酶提出的特异性抗体揭示了该酶在烟草茎维管组织中的积累，按照在木质化中的假定作用。为了进行功能分析，在拟南芥和N.通过RNA介导的转录后基因沉默来抑制本塞姆氏菌。在拟南芥中，基因沉默导致矮化表型和木质素组成的变化，如组织化学染色所示。对沉默N.通过免疫学、组织化学和化学方法对本塞姆氏植物的研究揭示了酰基转移酶沉默对可溶性苯丙素类和木质素含量和组成的影响。特别是，记录到双辛基单元减少和对羟基苯基单元增加。酶免疫共聚焦显微镜显示酶的积累水平和木质素组成的血管细胞之间的相关性。这些结果证明了酰基转移酶在苯丙素类生物合成中的功能。
The hydroxyl group in the 3-position of the phenylpropanoid compounds is introduced at the level of coumarate shikimate/ quinate esters, whose synthesis implicates an acyltransferase activity. Specific antibodies raised against the recombinant tobacco (Nicotiana tabacum) acyltransferase revealed the accumulation of the enzyme in stem vascular tissues of tobacco, in accordance with a putative role in lignification. For functional analysis, the acyltransferase gene was silenced in Arabidopsis thaliana and N. benthamiana by RNA-mediated posttranscriptional gene silencing. In Arabidopsis, gene silencing resulted in a dwarf phenotype and changes in lignin composition as indicated by histochemical staining. An in-depth study of silenced N. benthamiana plants by immunological, histochemical, and chemical methods revealed the impact of acyltransferase silencing on soluble phenylpropanoids and lignin content and composition. In particular, a decrease in syringyl units and an increase in p-hydroxyphenyl units were recorded. Enzyme immunolocalization by confocal microscopy showed a correlation between enzyme accumulation levels and lignin composition in vascular cells. These results demonstrate the function of the acyltransferase in phenylpropanoid biosynthesis.