Erythrocyte arginase, pyrimidine 5'-nucleotidase (P5N), and deoxypyrimidine 5'-nucleotidase (dP5N) as indices of lead exposure.
Erythrocyte arginase, pyrimidine 5'-nucleotidase (P5N), and deoxypyrimidine 5'-nucleotidase (dP5N) as indices of lead exposure.
复制标题
红细胞精氨酸酶、嘧啶 5-核苷酸酶 (P5N) 和脱氧嘧啶 5-核苷酸酶 (dP5N) 作为铅暴露指标。
DOI：
10.1136/oem.43.6.387
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Stohs,SJ
中科院分区：
文献类型：
--
作者：
Cook,LR;Angle,CR;Stohs,SJ
本文比较了16名铅作业工人和14名年龄相匹配的对照者红细胞内三种酶--β-D-半胱氨酸酶、嘧啶5 ′-核苷酸酶（P_5N）和脱氧嘧啶5 ′-核苷酸酶（dP_5N）的活性与血铅（PbB）和未提取锌原卟啉（EP）浓度的关系。PbB为0.9-2.5 μ M（19-52微克/分升）的受试者以尿苷一磷酸（UMP）为底物时，P5 N活性为6.5 +/- 0.6 IU，显著低于（p <0.001）PbB为0.3-0.6 μ M（6-12微克/分升）的对照组的12.0 +/- 0.7 IU活性。以脱氧尿苷一磷酸（dUMP）或胸苷一磷酸为底物的红细胞dP 5 N和红细胞脱氢酶的平均活性未区分两组。ln PbB与P5 N和dP 5 N所选底物的相关系数分别为：UMP，r = -0.75; dUMP，r = -0.61; TMP，r =-0.23。ln PbB与酶活性的相关系数为-0.03。红细胞P5 N（UMBN）是PbB的重要相关物，相当于红细胞原卟啉。HPLC法测定红细胞尿铅活性是一种灵敏、快速的测定方法，似乎符合血铅浓度可靠的临床实验室指标的标准。
The activities of three erythrocyte (rbc) enzymes, arginase, pyrimidine 5'-nucleotidase (P5N), and deoxypyrimidine 5'-nucleotidase (dP5N), were compared in 16 lead workers and 14 age matched controls as correlates of blood lead (PbB) and unextracted zinc protoporphyrin (EP) concentrations. Subjects with PbB of 0.9-2.5 microM (19-52 micrograms/dl) had 6.5 +/- 0.6 IU of P5N activity with uridine monophosphate (UMP) as substrate, significantly less (p less than 0.001) than the 12.0 +/- 0.7 IU activity of controls with PbB 0.3-0.6 microM (6-12 micrograms/dl). The mean activity of rbc dP5N with either deoxyuridine monophosphate (dUMP) or thymidine monophosphate as substrate, and of rbc arginase, did not differentiate the two groups. The correlation coefficients of ln PbB with the selected substrates for P5N and dP5N were: UMP, r = -0.75; dUMP, r = -0.61; TMP, r = -0.23. The correlation coefficient of ln PbB and arginase activity was -0.03. Rbc P5N (UMPase) is a significant correlate of PbB, equivalent to rbc protoporphyrin. HPLC assay of rbc UMPase activity is a sensitive and rapid assay that appears to meet criteria for a reliable clinical laboratory index of blood lead concentrations.