Oxidized low-density lipoproteins delay endothelial wound healing: lack of effect of vitamin E.
Oxidized low-density lipoproteins delay endothelial wound healing: lack of effect of vitamin E.
复制标题
氧化低密度脂蛋白延迟内皮伤口愈合：缺乏维生素E的作用。
DOI：
10.1159/000177836
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发表时间：
1995
影响因子：
3.9
通讯作者：
Chung,BH
中科院分区：
文献类型：
--
作者：
Boissonneault,GA;Wang,Y;Chung,BH
本研究的目的是检查氧化低密度脂蛋白（oxLDL）在体外创伤模型中对内皮再生的影响以及维生素E（E）可能提供的保护。通过刮擦来损伤生长在生物膜过滤器上的内皮细胞，并允许在LDL或oxLDL（25-200 µg/ml）、亚油酸（FA，90 µM）或亚油酸氢过氧化物（OFA，15 µM）存在下在裸露区域上重新建立生长24 h。一些单层细胞用25 µME预处理24 h。跨内皮白蛋白移动被用作内皮屏障功能的量度和内皮单层再生长的指标。暴露于低至25 μg/ml的oxLDL水平24小时导致内皮单层再生受到抑制，而天然LDL没有影响，并且用25 μME预富集没有提供保护。相比之下，E预富集改善内皮再生控制水平FA和OFA处理的文化，不像oxLDL处理的文化。结论：循环oxLDL可能减少损伤内皮的再生，补充E可能不提供保护。此外，脂肪酸或其氢过氧化物不太可能参与这种效应。
The purpose of this study was to examine the influence of oxidized low-density lipoprotein (oxLDL) on endothelial regrowth in an in vitro wounding model and the possible protection afforded by vitamin E (E). Endothelial cells grown on micropore filters were wounded by scraping and allowed to reestablish growth on denuded areas in the presence of LDL or oxLDL (25-200 µg/ml), linoleic acid (FA, 90 µM)or linoleic acid hydroperoxide (OFA, 15 µM)for 24 h. Some monolayers were pretreated with 25 µME for 24 h. Transendothelial albumin movement was used as a measure of endothelial barrier function and as an indicator of endothelial monolayer regrowth. Exposure to levels of oxLDL as low as 25 µg/ml for 24 h resulted in depressed endothelial monolayer regrowth, whereas native LDL was without effect and pre-enrichment with 25 µME offered no protection. In comparison, E preenrichment improved endothelial regrowth to control levels in FA- and OFA-treated cultures, unlike oxLDL-treated cultures. It is concluded that circulating oxLDL may reduce regrowth of wounded endothelium and supplemental E may not offer protection. Moreover, fatty acids or their hydroperoxides are unlikely to be involved in this effect.