Measurement of bacterivory by protists in open ocean waters
Measurement of bacterivory by protists in open ocean waters
复制标题
公海水域中原生生物的细菌测量
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
P. Burkill
中科院分区：
文献类型：
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作者：
M. Zubkov;M. Sleigh;P. Burkill
原生动物是水体中异养细菌的主要消费者。这些细菌和原生动物的数量在贫营养区的开放的海洋是低的，目前的方法缺乏敏感性，以评估率的噬菌性在这样的沃茨。本文提出了一种新的方法来估计双放射性标记的天然细菌的噬菌性，利用活的周围的猎物细菌和分离的捕食者从猎物。这种方法足够灵敏，可以在13小时的孵育期内测量不到1%的标记细菌的消耗。当测试从27个站在中营养和贫营养区域的北大西洋和南大西洋的样品，约17%的代谢活跃的细菌每天吃草，约60%的消耗的猎物生物量标记的14 C-亮氨酸被保留的捕食者。
Protozoa are the main consumers of heterotrophic bacteria in aquatic habitats. The numbers of these bacteria and protozoa in oligotrophic areas of the open ocean are low, and current methods lack the sensitivity to assess rates of bacterivory in such waters. A new method is proposed for estimating bacterivory on dual radioactively labelled natural bacteria using living ambient prey bacteria and separation of predators from prey by fractionation. This approach is sufficiently sensitive to measure the consumption of less than 1% of the labelled bacteria during a 13-h incubation period. When tested on samples collected from 27 stations in mesotrophic and oligotrophic regions of the North and South Atlantic Oceans, about 17% of metabolically active bacteria were grazed per day and about 60% of consumed prey biomass labelled with 14C-leucine was retained by the predators.