Glucose regulates proinsulin and prosomatostatin but not proglucagon messenger ribonucleic acid levels in rat pancreatic islets
Glucose regulates proinsulin and prosomatostatin but not proglucagon messenger ribonucleic acid levels in rat pancreatic islets
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DOI：
10.1210/en.141.1.174
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发表时间：
2000-01-01
期刊：
影响因子：
4.8
通讯作者：
Philippe, J
中科院分区：
文献类型：
--
作者：
Dumonteil, E;Magnan, C;Philippe, J
胰岛素和胰高血糖素是参与控制燃料代谢，特别是葡萄糖稳态的主要激素。反过来，营养物质严格调节胰岛的胰岛素和胰高血糖素分泌。营养素已被清楚地证明会影响胰岛素分泌、胰岛素生物合成和胰岛素原基因表达；相比之下，营养物质对胰高血糖素原基因表达的影响尚未研究。我们研究了葡萄糖、精氨酸和棕榈酸对 24 小时培养中分离的大鼠胰岛的胰高血糖素释放、胰高血糖素细胞含量和胰高血糖素原信使 RNA (mRNA) 水平的影响。我们在此报告，明确调节胰岛素和生长抑素释放以及胰岛素原和原生长抑素 mRNA 水平的葡萄糖浓度，不会显着影响胰高血糖素释放、胰高血糖素细胞含量或胰高血糖素原 mRNA 水平。此外，虽然 10 mM 精氨酸和 1 mM 棕榈酸盐都强烈刺激胰高血糖素释放，但它们并不影响胰高血糖素原 mRNA 水平。我们的结论是，与胰岛素和生长抑素相比，葡萄糖不影响胰高血糖素释放和胰高血糖素原 mRNA 水平，并且精氨酸和棕榈酸不协调调节胰高血糖素释放和胰高血糖素原 mRNA 水平。
Insulin and glucagon are the major hormones involved in the control of fuel metabolism and particularly of glucose homeostasis; in turn, nutrients tightly regulate insulin and glucagon secretion from the islets of Langerhans. Nutrients have clearly been shown to affect insulin secretion, as well as insulin biosynthesis and proinsulin gene expression; by contrast, the effects of nutrients on proglucagon gene expression have not been studied. We have investigated the effect of glucose, arginine, and palmitate on glucagon release, glucagon cell content, and proglucagon messenger RNA (mRNA) levels from isolated rat islets in 24-h incubations. We report here that concentrations of glucose that clearly regulate insulin and somatostatin release as well as proinsulin and prosomatostatin mRNA levels, do not significantly affect glucagon release, glucagon cell content or proglucagon mRNA levels. In addition, though both 10 mM arginine and 1 mM palmitate strongly stimulated glucagon release, they did not affect proglucagon mRNA levels. We conclude that, in contrast to insulin and somatostatin, glucose does not affect glucagon release and proglucagon mRNA levels, and arginine and palmitate do not coordinately regulate glucagon release and proglucagon mRNA levels.