Strand Invasion of Extended, Mixed-Sequence B-DNA by γPNAs
Strand Invasion of Extended, Mixed-Sequence B-DNA by γPNAs
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DOI：
10.1021/ja900228j
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发表时间：
2009-09-02
影响因子：
15
通讯作者：
Ly, Danith H.
中科院分区：
文献类型：
--
作者：
He, Gaofei;Rapireddy, Sirinivas;Ly, Danith H.
在这篇文章中，我们展示了长度为15-20个核苷酸的肽核酸（PNAs），当它们被预先组织成右旋螺旋时，可以侵入混合序列双螺旋B型DNA（B-DNA）。链侵入通过直接沃森-克里克碱基配对以高度序列特异性的方式发生。与先前开发的双链体侵入策略不同，其需要两条假互补PNA链同时结合到DNA，在这种情况下，仅需要γ PNA的单链进行侵入，并且不需要核碱基取代。
In this Communication, we show that peptide nucleic acids (PNAs) with lengths of 15-20 nucleotides, when preorganized into a right-handed helix, can invade mixed-sequence double-helical B-form DNA (B-DNA). Strand invasion occurs in a highly sequence-specific manner through direct Watson-Crick base pairing. Unlike the previously developed double-duplex invasion strategy, which requires simultaneous binding of two strands of pseudocomplementary PNAs to DNA, only a single strand of gamma PNA is required for invasion in this case, and no nucleobase substitution is needed.