Multiplane imaging and three dimensional nanoscale particle tracking in biological microscopy
Multiplane imaging and three dimensional nanoscale particle tracking in biological microscopy
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DOI：
10.1364/oe.18.000877
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发表时间：
2010-01-18
期刊：
影响因子：
3.8
通讯作者：
Greenaway, Alan H.
中科院分区：
文献类型：
--
作者：
Dalgarno, Paul A.;Dalgarno, Heather I. C.;Greenaway, Alan H.
传统的显微镜只能从单一的物体平面上产生清晰的图像。这通常是一个限制，特别是在细胞生物学中。我们提出了一种显微镜附件，它可以同时记录来自多个物体平面的清晰图像。关键的概念是在光学系统中引入一个扭曲的衍射光栅，建立一个依赖于顺序的聚焦功率，以产生多个图像，每个图像来自不同的物体平面。我们利用这种多平面成像不仅用于生物成像，还用于纳米粒子跟踪，通过使用图像清晰度度量参数化图像，实现类似于10 nm的z位置分辨率。(C)2010年美国光学学会
A conventional microscope produces a sharp image from just a single object-plane. This is often a limitation, notably in cell biology. We present a microscope attachment which records sharp images from several object-planes simultaneously. The key concept is to introduce a distorted diffraction grating into the optical system, establishing an order-dependent focussing power in order to generate several images, each arising from a different object-plane. We exploit this multiplane imaging not just for bio-imaging but also for nano-particle tracking, achieving similar to 10 nm z position resolution by parameterising the images with an image sharpness metric. (C)2010 Optical Society of America