Promoter Variation and Gene Expression of mcr-1-Harboring Plasmids in Clinical Isolates of Escherichia coli and Klebsiella pneumoniae from a Chinese Hospital
Promoter Variation and Gene Expression of mcr-1-Harboring Plasmids in Clinical Isolates of Escherichia coli and Klebsiella pneumoniae from a Chinese Hospital
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中国某医院临床分离的大肠杆菌和肺炎克雷伯菌中携带mcr-1的质粒的启动子变异和基因表达
DOI：
10.1128/aac.00018-18
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发表时间：
2018-05-01
影响因子：
4.9
通讯作者：
Chen, Liang
中科院分区：
文献类型：
--
作者：
Huang, Bin;He, Yuting;Chen, Liang
摘要对从中国一家三级医院收集的6株携带mcr-1的大肠埃希菌和肺炎克雷伯菌分离株进行下一代测序，发现mcr-1基因侧翼区域存在显著的序列变异。虽然序列变异显著影响mcr-1的表达和启动子活性，但mcr-1基因表达水平与体外粘菌素耐药水平无关，这需要进一步深入研究。
ABSTRACT Next-generation sequencing of 6 mcr-1-harboring Escherichia coli and Klebsiella pneumoniae isolates collected from a tertiary care hospital in China revealed significant sequence variations in the regions flanking the mcr-1 gene. While sequence variations significantly affected the expression and promoter activity of mcr-1, the mcr-1 gene expression levels did not correlate with the in vitro colistin resistance levels, which warrants further in-depth investigations.