Differential effects of transforming avian RNA tumor viruses on avian macrophages.
Differential effects of transforming avian RNA tumor viruses on avian macrophages.
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转化禽RNA肿瘤病毒对禽巨噬细胞的不同影响。
DOI：
10.1073/pnas.78.6.3600
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发表时间：
1981
影响因子：
11.1
通讯作者：
Boettiger,D
中科院分区：
文献类型：
--
作者：
Durban,EM;Boettiger,D
功能分化的鸡巨噬细胞是通过胚胎卵黄囊细胞体外分化获得的，并通过几种巨噬细胞特异性标记物进行鉴定。将这些巨噬细胞暴露于禽成肌细胞病病毒（AMV）、禽髓细胞瘤病病毒（MC29）、髓细胞病相关病毒（mav2）和劳斯肉瘤病毒布拉格株（PR-B RSV）后，获得均匀的、感染的、产生病毒的培养物。AMV和MC29都诱导了由这些病毒株诱导的典型体内白血病的形态转化。对这两种转化细胞类型中巨噬细胞特异性标记表达的分析表明，成熟巨噬细胞的不同标记被每种病毒抑制，尽管在转化事件之前的亲本细胞在两种情况下都是成熟的巨噬细胞。感染PR-B RSV和mav2的细胞与未感染的巨噬细胞在形态特征、生长速度或巨噬细胞分化功能的表达方面没有明显差异。该系统展示了一种细胞类型，它可以产生传染性的、转化的RSV，但对转化基因src诱导的功能改变没有反应。
Functionally differentiated chicken macrophages were derived by in vitro differentiation of embryonic yolk sac cells and were characterized by several macrophage-specific cell markers. Uniform, infected, virus-producing cultures were obtained after exposure of these macrophages to avian myoblastosis virus (AMV), avian myelocytomatosis virus (MC29), myeloblastosis-associated virus (MAV-2), and Prague strain of Rous sarcoma virus (PR-B RSV). Both AMV and MC29 induced morphological transformation typical of the in vivo leukemias induced by these virus strains. Analysis of the expression of macrophage-specific markers in these two transformed cell types demonstrated that different markers of the mature macrophage were suppressed by each virus, even though the parental cell immediately preceding the transformation event was a mature macrophage in both cases. Cells infected with PR-B RSV and MAV-2 showed no observable difference from uninfected macrophages in terms of morphological characteristics, growth rate, or expression of the differentiated functions of macrophages. Ths system provides demonstrations of a cell type that produces infectious, transforming RSV but fails to respond by functional alterations induced by the transforming gene, src.