Combined preparation of ferredoxin, ferredoxin-NADP+ reductase and plastocyanin from spinach leaves.
Combined preparation of ferredoxin, ferredoxin-NADP+ reductase and plastocyanin from spinach leaves.
复制标题
菠菜叶中铁氧还蛋白、铁氧还蛋白-NADP还原酶和质体蓝素的联合制备。
DOI：
10.1016/0005-2728(70)90010-1
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发表时间：
1970
期刊：
影响因子：
--
通讯作者：
J. Wessels
中科院分区：
文献类型：
--
作者：
M. Borchert;J. Wessels
建立了一种从菠菜叶片中大规模联合制备铁氧还蛋白、铁还蛋白-NADP+还原酶(NADPH：铁氧还蛋白氧化还原酶，EC 1.6.99.4)和植物绿素的工艺。通过对单个酶的常规分离程序的修改、简化和调整，证明了以高纯度和高产量获得这些蛋白质是可能的。
A large-scale procedure has been developed for the combined preparation of ferredoxin, ferredoxin-NADP+reductase (NADPH:ferredoxin oxidoreductase, EC 1.6.99.4) and plastocyanin from spinach leaves. By modification, simplification and adjustment of common isolation procedures for the individual enzymes, it has proved possible to obtain these proteins in a highly purified state and in good yield.