Molecular Characterization of Astrovirus Infection in Children With Diarrhea in Beijing, 2005-2007
Molecular Characterization of Astrovirus Infection in Children With Diarrhea in Beijing, 2005-2007
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DOI：
10.1002/jmv.21729
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发表时间：
2010-03-01
影响因子：
12.7
通讯作者：
Hung, Tao
中科院分区：
文献类型：
--
作者：
Guo, Li;Xu, Xiwei;Hung, Tao
人类星状病毒（HAstV）已被认为是儿童急性胃肠炎的主要原因之一。为了更深入地了解中国HAstV胃肠炎的流行情况，我们从2005年3月至2007年11月期间采集了北京急性胃肠炎儿童的664份粪便样本，并对样本进行了基因分析。在该研究中，使用针对 ORF2 区域的 RT-PCR 检测对 HAstV 的所有八种血清型（基因型）进行了筛选。检测结果在 52 名 (7.8%) 患者中检测到了 HAstV，其中 HAstV-1 (50/52) 是研究期间的主要基因型。还检测到两种次要基因型 HAstV-6 和 HAstV-3。对50个HAstV-1毒株的部分测序表明，这些毒株之间ORF1a区核苷酸序列的同源性为88.4-100％，而氨基酸序列的同源性为95.6-100％。在部分区域中，核苷酸同一性范围为91.5%至100%，氨基酸同一性范围为97.3%至100%。随机检查的四个HAstV-1毒株之间的全基因组序列同一性为91-99%。在本研究中，HAstV 中未观察到重组事件。这项研究的结果将为 HAstV 监测和控制提供基线数据。 J. Med。病毒。 82:415-423, 2010。(C) 2010 Wiley-Liss, Inc.
Human astroviruses (HAstVs) have been recognized as one of the major causes of acute gastroenteritis in children. To provide more insight into the prevalence of HAstV gastroenteritis in China, 664 fecal samples were collected from children affected with acute gastroenteritis in Beijing from March 2005 to November 2007. The samples were analyzed genetically. All eight serotypes (genotypes) of HAstVs were screened using RT-PCR assays targeting the ORF2 region in the study. The assays detected HAstVs in 52 (7.8%) of the patients, with HAstV-1 (50/52) being the dominant genotype during the study period. Two minor genotypes, HAstV-6 and HAstV-3, were also detected. Partial sequencing of the 50 HAstV-1 strains showed that the homology of the nucleotide sequence of the ORF1a region between these strains was 88.4-100%, whereas the homology of the amino acid sequences was 95.6-100%. In the partial region, the nucleotide identities ranged from 91.5% to 100%, and amino acid identities ranged from 97.3% to 100%. The identity of the whole genome sequence between four randomly examined HAstV-1 strains was 91-99%. No recombination events were observed in HAstVs in this study. The findings of this study will provide baseline data for HAstVs surveillance and control. J. Med. Virol. 82:415-423, 2010. (C) 2010 Wiley-Liss, Inc.