A T4 bacteriophage mutant which lacks deoxyribonucleic acid polymerase but retains the polymerase-associated nuclease.
A T4 bacteriophage mutant which lacks deoxyribonucleic acid polymerase but retains the polymerase-associated nuclease.
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一种 T4 噬菌体突变体，缺乏脱氧核糖核酸聚合酶，但保留了聚合酶相关核酸酶。
DOI：
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发表时间：
1969
影响因子：
4.8
通讯作者：
N. G. Nossal
中科院分区：
文献类型：
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作者：
N. G. Nossal
摘要43号基因中的许多T4噬菌体琥珀突变体既不能诱导噬菌体DNA聚合酶的合成，也不能诱导物理上离不开聚合酶的核酸外切酶的合成。已经发现了一个突变体，其中43基因的单个琥珀突变导致产生一种蛋白质，该蛋白质没有聚合酶，但保留了野生型酶的核酸酶功能。该突变体在两个不相关的非允许宿主中形成的酶具有与野生型略有不同的层析和酶性质。该突变体的一步回复突变和允许宿主的感染导致聚合酶和相关的核酸酶，其层析和酶学性质接近野生型。
Abstract Many T4 bacteriophage amber mutants in gene 43 do not induce the synthesis of either the phage DNA polymerase or the exonuclease which is physically inseparable from the polymerase. One mutant has been found in which a single amber mutation in gene 43 leads to the production of a protein which lacks the polymerase but retains the nuclease function of the wild type enzyme. The enzyme formed by this mutant in two unrelated nonpermissive hosts has chromatographic and enzymatic properties which differ somewhat from the wild type. Single step revertants of this mutant and infection of a permissive host by the mutant result in a polymerase and associated nuclease whose chromatographic and enzymatic properties are close to that of the wild type.