Smooth muscle actin and myosin expression in cultured airway smooth muscle cells.
Smooth muscle actin and myosin expression in cultured airway smooth muscle cells.
复制标题
培养的气道平滑肌细胞中平滑肌肌动蛋白和肌球蛋白的表达。
DOI：
10.1152/ajplung.1998.274.5.l786
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Low,RB
中科院分区：
文献类型：
--
作者：
Wong,JZ;Woodcock-Mitchell,J;Mitchell,J;Rippetoe,P;White,S;Absher,M;Baldor,L;Evans,J;McHugh,KM;Low,RB
在这项研究中，平滑肌肌动蛋白和肌球蛋白的表达在大鼠气管平滑肌细胞培养进行了检查。蛋白质和mRNA分析表明，这些细胞表达α-和γ-平滑肌肌动蛋白和平滑肌肌球蛋白和非肌肉肌球蛋白B重链。当生长条件改变时，平滑肌特异性肌动蛋白和肌球蛋白亚型的表达以相同的方向调节。因此，在含1或10%血清的培养基中汇合时以及对于缺乏血清的低密度细胞（50-60%汇合），平滑肌形式的肌动蛋白和肌球蛋白的表达相对较高。相反，在10%血清中低密度的快速增殖培养物中，平滑肌收缩蛋白表达较低。非肌肉肌球蛋白B的mRNA和蛋白的表达是更稳定的，只有在生长的细胞在很小程度上上调。我们的研究结果为气道平滑肌细胞分化和收缩功能的分子基础提供了新的见解。
In this study, the expression of smooth muscle actin and myosin was examined in cultures of rat tracheal smooth muscle cells. Protein and mRNA analyses demonstrated that these cells express α- and γ-smooth muscle actin and smooth muscle myosin and nonmuscle myosin-B heavy chains. The expression of the smooth muscle specific actin and myosin isoforms was regulated in the same direction when growth conditions were changed. Thus, at confluency in 1 or 10% serum-containing medium as well as for low-density cells (50–60% confluent) deprived of serum, the expression of the smooth muscle forms of actin and myosin was relatively high. Conversely, in rapidly proliferating cultures at low density in 10% serum, smooth muscle contractile protein expression was low. The expression of nonmuscle myosin-B mRNA and protein was more stable and was upregulated only to a small degree in growing cells. Our results provide new insight into the molecular basis of differentiation and contractile function in airway smooth muscle cells.