Possible emergence of new geminiviruses by frequent recombination
Possible emergence of new geminiviruses by frequent recombination
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DOI：
10.1006/viro.1999.0056
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发表时间：
1999-12-20
期刊：
影响因子：
3.7
通讯作者：
Fauquet, CM
中科院分区：
文献类型：
--
作者：
Padidam, M;Sawyer, S;Fauquet, CM
虽然通过重组交换遗传信息在病毒进化中起着重要作用，但它在多大程度上产生了多样性尚不清楚。我们分析了基因组的双生病毒重组使用一种新的统计方法来检测基因转换。双生病毒（Geminiviruses）是双生病毒科（Geminiviridae）的一组植物病毒，其特征在于环状单链DNA（长度约2700个核苷酸）的基因组以孪生准等距颗粒的形式存在。对双生病毒的完整核苷酸序列进行比对，并通过基于多态性位点的随机分布搜索比预期更相似的序列的病毒对来检测重组事件。分析表明，重组是非常频繁的，发生在物种之间和属内和跨属。测试发现了分布在基因组中的420个具有统计学意义的重组片段。结果表明，重组是双生病毒进化的重要贡献者。高重组率可能是最近出现新的双生病毒疾病的原因。(C)北京：科学出版社.
Although exchange of genetic information by recombination plays a role in the evolution of viruses, the extent to which it generates diversity is not clear. We analyzed genomes of geminiviruses for recombination using a new statistical procedure developed to detect gene conversions. Geminiviruses (family, Geminiviridae) are a group of plant viruses characterized by a genome of circular single-stranded DNA (similar to 2700 nucleotides in length) encapsidated in twinned quasi-isometric particles. Complete nucleotide sequences of geminiviruses were aligned, and recombination events were detected by searching pairs of viruses for sequences that are significantly more similar than expected based on random distribution of polymorphic sites. The analyses revealed that recombination is very frequent and occurs between species and within and across genera. Tests identified 420 statistically significant recombinant fragments distributed across the genome. The results suggest that recombination is a significant contributor to geminivirus evolution. The high rate of recombination may he contributing to the recent emergence of new geminivirus diseases. (C) 1999 Academic Press.