Age-dependent deamidation of asparagine residues in proteins
Age-dependent deamidation of asparagine residues in proteins
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DOI：
10.1016/s0531-5565(01)00140-1
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发表时间：
2001-09-01
影响因子：
3.9
通讯作者：
Helliger, W
中科院分区：
文献类型：
--
作者：
Lindner, H;Helliger, W
多肽和蛋白质的非酶脱酰胺化是细胞在体外分离或储存过程中以及体内细胞发育和/或老化过程中发生的一种重要的降解反应。本文首先概述了结构对由五元琥珀酰亚胺中间体进行的脱酰胺反应的影响，然后概述了脱胺形式的分离和检测方法。对体外和体内脱酰胺化的实例进行了综述，包括这种蛋白质降解可能产生的生物学后果。最后，阐明了蛋白质甲基转移酶与L-异天冬氨酸和D-天冬氨酸残基的反应及其在蛋白质修复中的可能作用。(C)2001 Elsevier Science Inc.保留所有权利。
Nonenzymatic deamidation of peptides and proteins represents an important degradation reaction occurring in vitro in the course of isolation or storage and in vivo during development and/or aging of cells. This review first presents a synopsis of the influence of structure on deamidation reaction proceeding via a five-membered succinimide intermediate, followed by an outline of procedures for separation and detection of deamidated forms. Selected examples for in vitro and in vivo deamidation are reviewed including the possible biological consequences of this protein degradation. Finally, the reaction of protein methyltransferase with L-isoaspartyl- and D-aspartyl residues and its possible role in protein repair is elucidated. (C) 2001 Elsevier Science Inc. All rights reserved.