Physiological adaptation of Corynebacterium glutamicum to benzoate as alternative carbon source - a membrane proteome-centric view
Physiological adaptation of Corynebacterium glutamicum to benzoate as alternative carbon source - a membrane proteome-centric view
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DOI：
10.1002/pmic.200900025
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发表时间：
2009-07-01
期刊：
影响因子：
3.4
通讯作者：
Poetsch, Ansgar
中科院分区：
文献类型：
--
作者：
Haussmann, Ute;Qi, Su-Wei;Poetsch, Ansgar
微生物同化芳香族物质作为替代碳源的能力是天然芳香族化合物以及工业芳香族化合物生物降解的基础。在这项研究中，谷氨酸棒杆菌生长在苯甲酸盐作为唯一的碳源和能源。为了扩展在这一细胞室中发生的生理适应过程的稀缺知识，膜蛋白质组研究定量和定性方面，应用鸟枪蛋白质组学，以达到全面的调查。使用用N-15代谢标记的内标物相对定量膜蛋白。总之，40种蛋白质被发现在苯甲酸盐的生长过程中改变其丰度相比，葡萄糖。在苯甲酸盐生长的细胞的膜中观察到一个全球性的适应，其特征在于呼吸链的蛋白质丰度增加，饥饿反应，以及涉及调节剂McbR的硫代谢的变化。除了相对定量外，稳定同位素标记的合成肽用于绝对定量C. glutamicum、BenK和贝内.结果发现，这两种转运蛋白在苯甲酸盐的生长过程中表达，表明这两种转运蛋白都对苯甲酸盐的摄取有很大贡献。
The ability of microorganisms to assimilate aromatic substances as alternative carbon sources is the basis of biodegradation of natural as well as industrial aromatic compounds. In this study, Corynebacterium glutamicum was grown on benzoate as sole carbon and energy source. To extend the scarce knowledge about physiological adaptation processes occurring in this cell compartment, the membrane proteome was investigated under quantitative and qualitative aspects by applying shotgun proteomics to reach a comprehensive survey. Membrane proteins were relatively quantified using an internal standard metabolically labeled with N-15. Altogether, 40 proteins were found to change their abundance during growth on benzoate in comparison to glucose. A global adaptation was observed in the membrane of benzoate-grown cells, characterized by increased abundance of proteins of the respiratory chain, by a starvation response, and by changes in sulfur metabolism involving the regulator McbR. Additional to the relative quantification, stable isotope-labeled synthetic peptides were used for the absolute quantification of the two benzoate transporters of C. glutamicum, BenK and BenE. It was found that both transporters were expressed during growth on benzoate, suggesting that both contribute substantially to benzoate uptake.