Relationship between calcium, cyclic AMP, ATP, and intracellular pH and the capacity of hamster spermatozoa to express hyperactivated motility.
Relationship between calcium, cyclic AMP, ATP, and intracellular pH and the capacity of hamster spermatozoa to express hyperactivated motility.
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钙、环磷酸腺苷、ATP 和细胞内 pH 值与仓鼠精子表达过度活跃运动能力之间的关系。
DOI：
10.1002/mrd.1120220205
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
R. John Aitken
中科院分区：
文献类型：
--
作者：
David R. White;R. John Aitken
仓鼠尾端精子在1 × 10(6)/ml密度下的获能与cAMP水平的进行性升高有关，cAMP水平在运动过度激活发生之前升高。在相同条件下培养的头精子不表现出这种增加。在缺乏外源性钙的情况下，过度活化的发生率和cAMP水平的升高都严重减弱。添加磷酸二酯酶抑制剂IBMX后，这两个因子都不能恢复到控制水平，尽管在外源性钙的存在下，该试剂增加了cAMP水平，刺激了百分比运动，并促进了过度激活的出现。用钙调素拮抗剂卡咪达唑（calmidazolium， CZ）以1 × 10(6)/ml的密度处理精子，导致精子活力严重破坏，并消除过度激活，而cAMP含量仅轻微降低。在CZ存在的情况下，添加IBMX使cAMP含量升高到比正常情况下观察到的水平，但没有恢复协调或过度激活的运动。为了确定这种cAMP含量升高的机制，以及在附睾成熟过程中发生的变化，以促进这种增加的表达，比较了游离胞质钙浓度、ATP水平和头尾细胞的细胞内pH。随着cAMP水平的升高，尾端精子钙含量显著升高，而ATP含量和细胞内pH值下降。然而，头精子不能表达cAMP含量的增加并不是因为这些因素中的任何一个缺乏。这些结果表明，cAMP的升高在过度激活运动的表达中起着积极的作用，并且控制这两种事件的基本控制机制可能是在该物种中伴随着能化的钙的流入。
Capacitation of hamster caudal spermatozoa at a density of 1 x 10(6)/ml is associated with a progressive rise in cAMP levels that precedes the onset of hyperactivated motility. This increase is not expressed by caput spermatozoa incubated under identical conditions. Both the incidence of hyperactivation and the rise in cAMP levels are severely attenuated in the absence of exogenous calcium. Neither factor is restored to control levels by the addition of the phosphodiesterase inhibitor IBMX, although in the presence of exogenous calcium, this reagent increased cAMP levels, stimulated percentage motility and advanced the appearance of hyperactivation. Treatment of spermatozoa at a density of 1 x 10(6)/ml with the calmodulin antagonist, calmidazolium (CZ), caused severe disruption of sperm motility and abolished hyperactivation, while causing only a slight reduction in cAMP content. Addition of IBMX in the presence of CZ elevated cAMP content to levels higher than normally observed during capacitation but did not restore either coordinated or hyperactivated motility. To determine both the mechanisms responsible for this elevation of cAMP content and the changes that occur during epididymal maturation to facilitate the expression of this increase, the free cytosolic calcium concentration, ATP levels, and intracellular pH of caput and caudal cells were compared. The calcium content of caudal spermatozoa rose significantly at a time when cAMP levels were increasing, while ATP content and intracellular pH fell. However, the inability of caput spermatozoa to express a rise in cAMP content was not due to deficiencies in any of these factors. These results indicate a positive role for the cAMP rise in the expression of hyperactivated motility and that the fundamental control mechanism governing both these events may be the influx of calcium that accompanies capacitation in this species.
speract 和莫能菌素对海胆精子的钠依赖性激活。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Hansbrough,JR;Garbers,DL
通讯作者：
Garbers,DL