CORRELATION OF QUANTITATIVE CHANGES OF GASTRIC-MUCOSAL GLYCOPROTEINS WITH ASPRIN-INDUCED GASTRIC DAMAGE IN RATS
CORRELATION OF QUANTITATIVE CHANGES OF GASTRIC-MUCOSAL GLYCOPROTEINS WITH ASPRIN-INDUCED GASTRIC DAMAGE IN RATS
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DOI：
10.1136/gut.21.6.533
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发表时间：
1980-01-01
期刊：
影响因子：
24.5
通讯作者：
HOTTA, K
中科院分区：
文献类型：
--
作者：
AZUUMI, Y;OHARA, S;HOTTA, K
本文研究了乙酰水杨酸（阿司匹林）致大鼠胃粘膜损伤时胃粘膜糖蛋白的定量变化。口服阿司匹林后1 h内，肉眼观察到胃损伤。最显著的变化发生在给药后5 h，损伤在给药后9 h内被克服。用含TritonX-100的Tris缓冲液从大鼠胃中提取糖蛋白，经Bio-GelA-1.5m柱层析分离，分成3个组分。第一个峰对应于含或不含硫酸盐的胃粘液大分子中性和酸性糖蛋白（Fr.I），在阿司匹林给药后减弱。一个相当大的变化的Fr.I（49%的控制）出现在3小时，并逐渐恢复到控制值随后观察。糖蛋白含量的变化先于粘膜的宏观变化。阿司匹林引起的胃溃疡可能是由于胃粘液大分子糖蛋白缺乏所致。
Quantitative changes of gastric mucosal glycoproteins with the gastric damage induced by acetylsalicylic acid (aspirin) in rat were studied. Gastric injury was easily observed macroscopically within 1 h after the oral administration of aspirin. The most striking changes occurred at 5 h and the injury was overcome within 9 h after dosing. The glycoproteins extracted from rat stomach with Tris buffer containing Triton X-100 were fractionated on Bio-Gel A-1.5 m column chromatography and divided into 3 fractions. The 1st peak, corresponding to gastric mucus macromolecular neutral and acidic glycoproteins with or without sulfate (Fr.I), was diminished after aspirin administration. A considerable alteration of Fr.I (49% of control) appeared at 3 h and a gradual return to the control value was observed subsequently. The changes in the amount of glycoproteins were detected before the macroscopical changes of the mucosa. Gastric ulceration induced by aspirin may be caused by a deficiency of gastric mucus macromolecular glycoproteins of gastric mucus.