Transplantation of germ cells from rabbits and dogs into mouse testes
Transplantation of germ cells from rabbits and dogs into mouse testes
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DOI：
10.1095/biolreprod61.5.1331
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发表时间：
1999-11-01
影响因子：
3.6
通讯作者：
Brinster, RL
中科院分区：
文献类型：
--
作者：
Dobrinski, I;Avarbock, MR;Brinster, RL
将可育小鼠的精原干细胞移植到不育小鼠的曲细精管中，可以发育精子发生并将供体单倍型传递给受体小鼠的后代。当将大鼠或仓鼠的睾丸细胞移植到免疫缺陷小鼠的睾丸中时，受体小鼠睾丸中发生了完整的大鼠或仓鼠精子发生，尽管仓鼠的效率较低。本研究的目的是探讨供体和受体动物之间的系统发育距离增加对精原细胞移植结果的影响。从供体兔和犬收集睾丸细胞，移植到内源性精子发生被破坏的免疫缺陷受体小鼠的睾丸中。在单独的实验中，将兔或狗睾丸细胞冷冻并储存在液氮中或在移植到小鼠之前培养1个月。在移植后1至> 12个月，使用供体特异性多克隆抗体分析睾丸中供体生殖细胞的存在。此外，使用犬α-卫星DNA特异性引物通过聚合酶链反应证明受体睾丸中存在犬细胞，除一名受体外，所有受体的睾丸中均存在供体生殖细胞。供体生殖细胞主要形成由细胞间桥连接的圆形细胞链和网络，但未观察到供体来源的精子发生的后期阶段。这些结果表明，新鲜和冷冻保存的生殖细胞可以定殖在小鼠睾丸，但不能分化超过精原细胞的扩展阶段。
Spermatogonial stem cells of a fertile mouse transplanted into the seminiferous tubules of an infertile mouse can develop spermatogenesis and transmit the donor haplotype to progeny of the recipient mouse. When testis cells from rats or hamsters were transplanted to the testes of immunodeficient mice, complete rat or hamster spermatogenesis occurred in the recipient mouse testes, albeit with lower efficiency for the hamster. The objective of the present study was to investigate the effect of increasing phylogenetic distance between donor and recipient animals on the outcome of spermatogonial transplantation. Testis cells were collected from donor rabbits and dogs and transplanted into testes of immunodeficient recipient mice in which endogenous spermatogenesis had been destroyed. In separate experiments, rabbit or dog testis cells were frozen and stored in liquid nitrogen or cultured for 1 mo before transplantation to mice. Recipient testes were analyzed, using donor-specific polyclonal antibodies, from 1 to > 12 mo after transplantation for the presence of donor germ cells. In addition, the presence of canine cells in recipient testes was demonstrated by polymerase chain reaction using primers specific for canine alpha-satellite DNA, Donor germ cells were present in the testes of all but one recipient. Donor germ cells predominantly formed chains and networks of round cells connected by intercellular bridges, but later stages of donor-derived spermatogenesis were not observed. The pattern of colonization after transplantation of cultured cells did not resemble spermatogonial proliferation, These results indicate that fresh and cryopreserved germ cells can colonize the mouse testis but do not differentiate beyond the stage of spermatogonial expansion.