Genome-wide analysis of MATE transporters and expression patterns of a subgroup of MATE genes in response to aluminum toxicity in soybean.
Genome-wide analysis of MATE transporters and expression patterns of a subgroup of MATE genes in response to aluminum toxicity in soybean.
复制标题
对大豆中铝毒性反应的 MATE 转运蛋白和 MATE 基因亚组的表达模式进行全基因组分析
DOI：
10.1186/s12864-016-2559-8
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发表时间：
2016-03-11
期刊：
影响因子：
4.4
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Liu J;Li Y;Wang W;Gai J;Li Y
多药毒性化合物外排转运蛋白（MATE）家族是一类重要的多药外排转运蛋白，主要转运有机酸、植物激素和次生代谢产物等多种底物。然而，MATE家族在植物物种中的全基因组分析是有限的，在大豆中还没有这样的研究报道。从大豆（Glycine max）全基因组序列中共鉴定出117个MATE转运蛋白基因，命名为GmMATE 1-GmMATE 117。这117个GmMATE基因不均匀地定位在大豆染色体1至20上，同时检测到串联和节段重复事件，并且大多数基因表现出组织特异性表达模式。大豆MATE家族可分为4个亚家族，包括10个较小的亚家族，具有多种潜在功能，如类黄酮或生物碱的运输和积累、植物源或外源化合物的分泌、抗病性的调节以及对非生物胁迫的响应。进一步分析了8个与铝解毒和铁转运相关的MATE转运蛋白。在这8个GmMATE基因的上游序列中发现了ABRE、ARE、HSE、LTR、MBS等7个逆境应答顺式元件以及ART 1（Al resistance transcription factor 1）的顺式元件GGNVS。通过对这8个GmMATE基因在铝胁迫下的差异表达分析，初步确定GmMATE 75为大豆耐铝的候选基因，其相对转录本丰度在铝胁迫后6、12和24 h均有所增加，耐铝品种的相对转录本丰度比铝敏感品种的相对转录本丰度增加了数倍，这与以往报道的耐铝相关MATE基因一致。在大豆中共鉴定出117个MATE转运蛋白，并通过与已知植物MATE转运蛋白的系统发育分析，提出了它们的潜在功能。对8个大豆铝解毒/铁转运相关基因MATE的顺式元件和表达模式进行分析，确定GmMATE 75为大豆耐铝候选基因。本研究首次揭示了大豆MATE家族及其在大豆对非生物胁迫特别是铝毒胁迫响应中的作用。本文的在线版本（doi：10.1186/s12864-016-2559-8）包含补充材料，可供授权用户使用。
Multidrug and toxic compound extrusion (MATE) family is an important group of the multidrug efflux transporters that extrude organic compounds, transporting a broad range of substrates such as organic acids, plant hormones and secondary metabolites. However, genome-wide analysis of MATE family in plant species is limited and no such studies have been reported in soybean. A total of 117 genes encoding MATE transporters were identified from the whole genome sequence of soybean (Glycine max), which were denominated as GmMATE1 - GmMATE117. These 117 GmMATE genes were unevenly localized on soybean chromosomes 1 to 20, with both tandem and segmental duplication events detected, and most genes showed tissue-specific expression patterns. Soybean MATE family could be classified into four subfamilies comprising ten smaller subgroups, with diverse potential functions such as transport and accumulation of flavonoids or alkaloids, extrusion of plant-derived or xenobiotic compounds, regulation of disease resistance, and response to abiotic stresses. Eight soybean MATE transporters clustered together with the previously reported MATE proteins related to aluminum (Al) detoxification and iron translocation were further analyzed. Seven stress-responsive cis-elements such as ABRE, ARE, HSE, LTR, MBS, as well as a cis-element of ART1 (Al resistance transcription factor 1), GGNVS, were identified in the upstream region of these eight GmMATE genes. Differential gene expression analysis of these eight GmMATE genes in response to Al stress helps us identify GmMATE75 as the candidate gene for Al tolerance in soybean, whose relative transcript abundance increased at 6, 12 and 24 h after Al treatment, with more fold changes in Al-tolerant than Al-sensitive cultivar, which is consistent with previously reported Al-tolerance related MATE genes. A total of 117 MATE transporters were identified in soybean and their potential functions were proposed by phylogenetic analysis with known plant MATE transporters. The cis-elements and expression patterns of eight soybean MATE genes related to Al detoxification/iron translocation were analyzed, and GmMATE75 was identified as a candidate gene for Al tolerance in soybean. This study provides a first insight on soybean MATE family and their potential roles in soybean response to abiotic stresses especially Al toxicity. The online version of this article (doi:10.1186/s12864-016-2559-8) contains supplementary material, which is available to authorized users.