Cystic fibrosis transmembrane conductance regulator currents in guinea pig pancreatic duct cells: inhibition by bicarbonate ions.
Cystic fibrosis transmembrane conductance regulator currents in guinea pig pancreatic duct cells: inhibition by bicarbonate ions.
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豚鼠胰管细胞囊性纤维化跨膜电导调节电流：碳酸氢根离子的抑制。
DOI：
10.1016/s0016-5085(00)70372-6
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发表时间：
2000
期刊：
影响因子：
29.4
通讯作者：
M. Gray
中科院分区：
文献类型：
--
作者：
C. O'Reilly;J. Winpenny;B. Argent;M. Gray
背景与目的囊性纤维化跨膜电导调节剂ClHCO3通道在胰管细胞分泌−−中起重要作用。方法从小叶内和小叶间小导管分离PDCs，用全细胞膜片钳技术研究其氯-−电导。结果3‘，5’-环磷酸腺苷(CAMP)能激活1 1 4个细胞的−电导(5 6%)。在2 0 4个细胞中，有39个细胞(19%)激活了更大的(10倍)、时间和电压依赖的CL−电导。促胰液素也有类似的作用。在同一细胞中观察到两种电导的共表达，两种电导具有相似的阴离子选择性和药理作用。胞外HCO3−对两种电流(Ki，̃7 mmoL/L)均有剂量依赖性的抑制作用，且与细胞内外pH和洗浴溶液中PCO2和CO32−含量无关。结论cAMP可激活豚鼠PDCs两种不同的CL-−电导。因为这些电导是共表达的，并且表现出相似的特征(阴离子选择性、药理学和HCO3−抑制)，我们得出结论，CFtR是它们的基础。HCO3−对CFtR的抑制作用对目前的胰腺导管HCO3−分泌模型有一定的意义。胃肠病学2000；118：1187-1196
Background & AimsCystic fibrosis transmembrane conductance regulator (CFTR) Cl−channels play an important role in HCO3−secretion by pancreatic duct cells (PDCs). Our aims were to characterize the CFTR conductance of guinea pig PDCs and to establish whether CFTR is regulated by HCO3−.MethodsPDCs were isolated from small intralobular and interlobular ducts, and their Cl−conductance was studied using the whole-cell patch clamp technique.ResultsActivation of a typical CFTR conductance by adenosine 3',5'-cyclic monophosphate (cAMP) was observed in 114 of 204 cells (56%). A larger (10-fold), time- and voltage-dependent Cl−conductance was activated in 39 of 204 cells (19%). Secretin had a similar effect. Coexpression of both conductances in the same cell was observed, and both conductances had similar anion selectivity and pharmacology. Extracellular HCO3−caused a dose-dependent inhibition of both currents (Ki, ̃7 mmol/L), which was independent of intracellular and extracellular pH, and the PCO2and CO32−content of the bathing solutions. Conclusions Two kinetically distinct Cl−conductances are activated by cAMP in guinea pig PDCs. Because these conductances are coexpressed and exhibit similar characteristics (anion selectivity, pharmacology, and HCO3−inhibition), we conclude that CFTR underlies them both. The inhibition of CFTR by HCO3−has implications for the current model of pancreatic ductal HCO3−secretion. GASTROENTEROLOGY 2000;118:1187-1196
影响因子：
56.9
作者：
J. Riordan;J. Rommens;N. Alon;R. Rozmahel;Z. Grzelczak;J. Zieleński;N. Plavsic;Jia-Ling Chou
通讯作者：
J. Riordan;J. Rommens;N. Alon;R. Rozmahel;Z. Grzelczak;J. Zieleński;N. Plavsic;Jia-Ling Chou
培养的狗胰管上皮细胞中两种不同氯离子通道的表征。
DOI：
10.1152/ajpgi.1997.272.1.g172
发表时间：
1997
期刊：
The American journal of physiology.
影响因子：
--
作者：
Nguyen,TD;Koh,DS;Moody,MW;Fox,NR;Savard,CE;Kuver,R;Hille,B;Lee,SP
通讯作者：
Lee,SP
DOI：
10.1073/pnas.91.12.5340
发表时间：
1994-06-07
影响因子：
11.1
作者：
POULSEN, JH;FISCHER, H;MACHEN, TE
通讯作者：
MACHEN, TE