Influence of the Cryoprotective Agents Glycerol and Hydroxyethyl Starch on Red Blood Cell ATP and 2,3‐Diphosphoglyceric Acid Levels
Influence of the Cryoprotective Agents Glycerol and Hydroxyethyl Starch on Red Blood Cell ATP and 2,3‐Diphosphoglyceric Acid Levels
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冷冻保护剂甘油和羟乙基淀粉对红细胞 ATP 和 2,3-二磷酸甘油酸水平的影响
DOI：
10.1111/j.1423-0410.1992.tb01187.x
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发表时间：
1992
期刊：
影响因子：
2.7
通讯作者：
U. Nydegger
中科院分区：
文献类型：
--
作者：
I. C. Rittmeyer;U. Nydegger
由于羟乙基淀粉(HAES)用于体积替代治疗和作为冷冻红细胞(RBC)的冷冻保护剂，与甘油不同，这种化合物在输血前不需要从解冻细胞中进行劳动密集型移除。本文报道了甘油和HAES对红细胞有机磷酸盐ATP和2，3-二磷酸甘油酸(2，3-DPG)的影响。将20名健康供者(3名女性，17名男性)的CPD-A1稳定的红细胞分别冷冻在40%甘油或6%HAES中，分子量为20万。分别在-80°C(甘油)和-196°C(HAES)条件下，测定加入冷冻保护剂前CPD-A1红细胞和冷冻解冻后24 h红细胞中ATP和2，3-DPG的含量。似乎HAES，但不是甘油，显著降低了ATP浓度，而两者都导致了2，3-DPG浓度的降低；这种减少在甘油中比在HAES中更明显。对6名献血者血液的实验表明，与甘油相比，HAES对自体溶血的影响为16%，而冷冻保护剂对自体溶血的影响仅为3.1%。使用甘油或HAES作为冷冻保护剂时，RBC回收率与之相当。出现了一种明显的模式，甘油降低2，3-DPG水平，HAES降低更少，HAES降低ATP水平，但甘油不降低。如果HAES作为一种方便的冷冻保护剂被引入，我们的发现可能是重要的。
Because hydroxyethyl starch (HAES) is used for volume replacement therapy and as a cryoprotectant for frozen red blood cells (RBCs), this compound, in contrast to glycerol, does not require labor‐intensive removal from thawed cells prior to transfusion. We here report the effect of both glycerol and HAES on the RBC organic phosphates ATP and 2,3‐diphosphoglyceric acid (2,3‐DPG). The CPD‐A1‐stabilized RBCs of 20 healthy donors (3 females, 17 males) were separately frozen in either 40% glycerol or 6% HAES, of molecular weight 200,000. ATP and 2,3‐DPG concentrations were determined in CPD‐A1 RBCs before addition of cryoprotectant and in cryopreserved thawed RBCs after 24 h storage at ‐80°C (glycerol) and ‐196°C (HAES). It appears that HAES, but not glycerol, significantly reduces ATP concentrations whereas both lead to a reduction of 2,3‐DPG concentrations; this reduction was more pronounced with glycerol than with HAES. Experiments with the blood of 6 donors demonstrated that HAES affects autohemolysis by 16%, in contrast to glycerol, after which cryoprotectant autohemolysis was affected by 3.1% only. RBC recoveries were comparable using glycerol or HAES as cryoprotectants. A distinct pattern of reduction of 2,3‐DPG levels by glycerol and less by HAES, and of ATP levels by HAES but not by glycerol, emerges. Our findings may be of importance if HAES is to be introduced as a convenient cryoprotectant.