Synthesis and Electro‐Optical Properties of a Novel DNA–Lipid Complex Carrying Carbazole Moieties
Synthesis and Electro‐Optical Properties of a Novel DNA–Lipid Complex Carrying Carbazole Moieties
复制标题
DOI：
10.1002/macp.200900397
复制
发表时间：
2010-02
影响因子：
2.5
通讯作者：
Jinqing Qu;Lujing Liu;Huan-qin Chen;N. Ogata;T. Masuda
中科院分区：
文献类型：
--
作者：
Jinqing Qu;Lujing Liu;Huan-qin Chen;N. Ogata;T. Masuda
以阳离子脂质（2Cz）取代钠抗衡阳离子，制备了一种新型的含咔唑（Cz）基团的DNA-脂质复合物，其中磷酸盐与脂质的实际摩尔比为1：1.05。DNA-脂质（2Cz）复合物可溶于CHCl 3、CH 2Cl 2、甲醇和乙醇等常见有机溶剂，而不溶于THF、甲苯和水。CD光谱显示，DNA-脂质复合物在甲醇中主要呈双螺旋结构，并且该螺旋结构对加热相当稳定。DNA-脂质（2Cz）复合物的溶液以40.0%的量子产率发射荧光，这低于相应的脂质（2Cz）的量子产率（76%）。复合物的循环伏安图表明DNA-脂质的氧化电位（2Cz）为0.65 V。根据空气中的TGA，DNA-脂质复合物的失重起始温度为230 °C。
A novel DNA―lipid complex carrying carbazole (Cz) moieties was prepared by substituting the sodium counter cation with cationic lipid, namely lipid(2Cz), in which the actual mole ratio of phosphate to lipid was 1:1.05. The DNA―lipid(2Cz) complex was soluble in common organic solvents including CHCl 3 , CH 2 Cl 2 , methanol, and ethanol, while insoluble in THF, toluene, and water. CD spectroscopy revealed that the DNA― lipid complex took a predominantly double helical structure in methanol and that the helical structure was fairly stable against heating. A solution of the DNA―lipid(2Cz) complex emitted fluorescence in 40.0% quantum yield, which was lower than that of the corresponding lipid(2Cz) (76%). The cyclic voltammograms of the complex indicated that the oxidation potential of DNA― lipid(2Cz) was 0.65 V. The onset temperature of weight loss of the DNA―lipid complex is 230 °C according to TGA in air.