MAPKKKα is a positive regulator of cell death associated with both plant immunity and disease
MAPKKKα is a positive regulator of cell death associated with both plant immunity and disease
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DOI：
10.1038/sj.emboj.7600283
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发表时间：
2004-08-04
期刊：
影响因子：
11.4
通讯作者：
Martin, GB
中科院分区：
文献类型：
--
作者：
del Pozo, O;Pedley, KF;Martin, GB
许多植物病原体引起疾病症状，表现为局部细胞死亡的区域。局部细胞死亡（过敏反应; HR）也发生在抗病植物中，但这种反应出现在试图感染的几个小时内，并可能限制病原体的进一步生长。我们鉴定了MAP激酶激酶激酶基因（MAPKKKa），该基因是HR和抗假单胞菌所需的。值得注意的是，我们发现MAPKKKa也调节易受感染的叶片中的细胞死亡。MAPKKKa α在叶片中的过表达激活了MAPKs并引起了病原体非依赖性细胞死亡。通过在叶片中过表达MAPKKKa，并通过病毒诱导的基因沉默抑制各种MAPKK和MAPK基因的表达，我们确定了两个不同的MAPK级联反应，其作用于MAPKKKa的下游。这些结果表明，与植物免疫和疾病易感性相关的信号转导途径共享一个共同的分子开关。
Many plant pathogens cause disease symptoms that manifest over days as regions of localized cell death. Localized cell death (the hypersensitive response; HR) also occurs in disease-resistant plants, but this response appears within hours of attempted infection and may restrict further pathogen growth. We identified a MAP kinase kinase kinase gene (MAPKKKalpha) that is required for the HR and resistance against Pseudomonas syringae. Significantly, we found that MAPKKKalpha also regulates cell death in susceptible leaves undergoing P. syringae infection. Overexpression of MAPKKKalpha in leaves activated MAPKs and caused pathogen-independent cell death. By overexpressing MAPKKKalpha in leaves and suppressing expression of various MAPKK and MAPK genes by virus-induced gene silencing, we identified two distinct MAPK cascades that act downstream of MAPKKKalpha. These results demonstrate that signal transduction pathways associated with both plant immunity and disease susceptibility share a common molecular switch.