Cloning, characterization, and localization of mouse and human SPO11
Cloning, characterization, and localization of mouse and human SPO11
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DOI：
10.1006/geno.1999.5955
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发表时间：
1999-10-15
期刊：
影响因子：
4.4
通讯作者：
Camerini-Otero, RD
中科院分区：
文献类型：
--
作者：
Romanienko, PJ;Camerini-Otero, RD
Spo 11是酵母中减数分裂特异性蛋白，已发现其在减数分裂的早期阶段与DNA双链断裂（DSB）共价结合。这些DSB启动同源重组，这是染色体正确分离和减数分裂期间产生遗传多样性所必需的。在这里，我们报告的克隆，表征，组织表达，和染色体定位的小鼠和人类的同源Spo 11。推定的小鼠和人类蛋白质是82%相同的，并与其他家族成员共享约25%的同一性。北方印迹分析表明，睾丸特异性表达的两个基因，但RT-PCR结果表明，普遍存在的至少一部分Spo 11在小鼠的表达。在几种体细胞组织中也检测到人SPO 11。小鼠Spo 11定位于染色体2 H4，人类SPO 11定位于染色体20q13.2-q13.3，这是在一些乳腺和卵巢肿瘤中扩增的区域。
Spo11 is a meiosis-specific protein in yeast that has been found covalently bound to DNA double-strand breaks (DSBs) during the early stages of meiosis. These DSBs initiate homologous recombination, which is required for proper segregation of chromosomes and the generation of genetic diversity during meiosis. Here we report the cloning, characterization, tissue expression, and chromosomal localization of both mouse and human homologues of Spo11. The putative mouse and human proteins are 82% identical and share approximately 25% identity with other family members. Northern blot analysis revealed testis-specific expression for both genes, but RT-PCR results showed ubiquitous expression of at least a portion of Spo11 in mouse. Human SPO11 was also detected in several somatic tissues. Mouse Spo11 was localized to chromosome 2H4, and human SPO11 was localized to chromosome 20q13.2-q13.3, a region amplified in some breast and ovarian tumors.