Regional and temporal regulation and role of somatostatin receptor subtypes in the mouse brain following systemic kainate-induced acute seizures
Regional and temporal regulation and role of somatostatin receptor subtypes in the mouse brain following systemic kainate-induced acute seizures
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DOI：
10.1016/j.neures.2019.01.004
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发表时间：
2019-12-01
影响因子：
2.9
通讯作者：
Tamura, Hideki
中科院分区：
文献类型：
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作者：
Iwasawa, Chizuru;Narita, Minoru;Tamura, Hideki
生长抑素通过生长抑素受体(SST(1)-SST(5))降低神经元兴奋性，抑制癫痫发作活动。然而，SST亚型在癫痫小鼠中的表达状况及其在生长抑素抗癫痫作用中的作用尚不清楚。在这里，我们展示了SST亚型受小鼠癫痫神经元活动的不同调节。注射海人藻氨酸后，大鼠海马区SST(2)和SST(3)基因表达迅速升高，SST(1)和SST(4)基因表达降低，而杏仁基底外侧核兴奋性神经元中只有SST(2)基因表达增加，同时SST(2)蛋白水平降低。给予红藻氨酸后，杏仁核基底外侧区SST(2)表达减少，延缓了癫痫的恢复，但在海马齿状回中未见SST(2)的表达；两种情况下，神经元活动标志物BDNF的表达水平都较高。这些结果表明，SST(2)在杏仁核通过反馈抑制参与癫痫的终止。这可能是治疗急性癫痫的潜在靶点。(C)2019年爱思唯尔公司和日本神经科学学会。版权所有。
Somatostatin reduces neuronal excitability via somatostatin receptors (Sst(1)-Sst(5)) and inhibits seizure activity. However, the expression status of the Sst subtypes in epileptic mice and their role in the antiepileptic effects of somatostatin remain unclear. Here, we show that the Sst subtypes are regulated differently by epileptic neuronal activity in mice. Systemic kainate injection rapidly and transiently elevated the Sst(2) and Sst(3) mRNA and reduced Sst(1) and Sst(4) mRNA in the hippocampus; however, among all the subtypes, only Sst(2) mRNA was increased in the excitatory neurons of the basolateral amygdala, accompanied by a decrease in the level of Sst(2) protein. Following kainate administration, recovery from seizure was delayed by reduced expression of Sst(2) in the basolateral amygdala, but not in the dentate gyrus of the hippocampus; higher expression levels of Bdnf, a neuronal activity marker, were observed in both conditions. These results suggest that Sst(2) contributes to seizure termination by feedback inhibition in the amygdala. This could be a potential therapeutic target for acute seizures. (C) 2019 Elsevier B.V. and Japan Neuroscience Society. All rights reserved.