Dehalococcoides ethenogenes strain 195 reductively dechlorinates diverse chlorinated aromatic pollutants
Dehalococcoides ethenogenes strain 195 reductively dechlorinates diverse chlorinated aromatic pollutants
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DOI：
10.1021/es034989b
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发表时间：
2004-04-01
影响因子：
11.4
通讯作者：
Häggblom, MM
中科院分区：
文献类型：
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作者：
Fennell, DE;Nijenhuis, I;Häggblom, MM
Dehalocococcoides ethenogenes菌株195将四氯乙烯脱氯为氯乙烯和乙烯，其基因组中含有多达17个推测的脱卤酶基因同源物，表明其具有不同的脱卤能力。我们修改了含有D.用1，2，3，4-四氯二苯并-对-二恶英、2，3，7，8-四氯二苯并-对-二恶英、2，3-二氯二苯并-对-二恶英、1，2，3，4-四氯二苯并呋喃、2，3，4，5，6-五氯联苯、1，2，3，4-四氯萘、各种氯苯或2-、3-和4-氯苯酚的混合物测定脱卤能力。D. ethenogenes菌株195将1，2，3，4-四氯二苯并-对-二恶英脱氯为1，2，4-三氯二苯并-对-二恶英和1，3-二氯二苯并-对-二恶英的混合物。2，3，4，5，6-五氯联苯脱氯后生成2，3，4，6-和/或2，3，5，6-四氯联苯和2，4，6-三氯联苯。1，2，3，4-四氯萘脱氯后主要生成一种未经鉴定的二氯萘同系物。六氯苯脱氯的主要最终产物是1，2，3，5-四氯苯和1，3，5-三氯苯。我们没有检测到一氯酚，2，3-二氯二苯并二恶英或2，3，7，8-四氯二苯并二恶英的脱氯子产物。D.除其已知的氯乙烯呼吸电子受体外，产乙烯菌菌株195还具有使许多不同类型的氯代芳族化合物脱氯的能力。在今后几年中，修复被多氯联苯和多氯二苯并对二恶英等芳香族卤代有机污染物污染的沉积物可能需要数十亿美元。利用微生物如Dehalococcoides spp.通过去除卤素使这些化合物解毒可能会导致成本效益高的生物技术补救方法。
Dehalococcoides ethenogenes strain 195 dechlorinates tetra chloroethene to vinyl chloride and ethene, and its genome has been found to contain up to 17 putative dehalogenase gene homologues, suggesting diverse dehalogenation ability. We amended pure or mixed cultures containing D. ethenogenes strain 195 with 1,2,3,4-tetrachlorodibenzo-p-dioxin, 2,3,7,8-tetrachlorodibenzo-p-dioxin, 2,3-dichlorodibenzo-p-dioxin, 1,2,3,4-tetrachlorodibenzofuran, 2,3,4,5,6-pentachlorobiphenyl, 1,2,3,4-tetra chloronaphthalene, various chlorobenzenes, or a mixture of 2-, 3-, and 4-chlorophenol to determine the dehalogenation ability. D. ethenogenes strain 195 dechlorinated 1,2,3,4-tetrachlorodibenzo-p-dioxin to a mixture of 1,2,4-trichlorodibenzo-p-dioxin and 1,3-dichlorodibenzo-p-dioxin. 2,3,4,5,6- Pentachlorobiphenyl was dechlorinated to 2,3,4,6- and/or 2,3,5,6-tetrachlorobiphenyl and 2,4,6-trichlorobiphenyl. 1,2,3,4-Tetrachloronaphthalene was dechlorinated primarily to an unidentified dichloro-naphthalene congener. The predominant end products from hexachlorobenzene dechlorination were 1,2,3,5-tetrachlorobenzene and 1,3,5-trichlorobenzene. We did not detect dechlorination daughter products from monochlorophenols, 2,3-dichlorodibenzo-p-dioxin or 2,3,7,8- tetrachlorodibenzop-dioxin. D. ethenogenes strain 195 has the ability to dechlorinate many different types of chlorinated aromatic compounds, in addition to its known chloroethene respiratory electron acceptors. Remediation of sediments contaminated with aromatic halogenated organic pollutants such as polychlorinated biphenyls and polychlorinated dibenzo-p-dioxins could require billions of dollars in the coming years. Harnessing microorganisms such as Dehalococcoides spp. that detoxify these compounds via removal of halogens may lead to cost-effective biotechnological approaches for remediation.