Creating genetically modified pigs by using nuclear transfer.
Creating genetically modified pigs by using nuclear transfer.
复制标题
DOI：
10.1186/1477-7827-1-82
复制
发表时间：
2003-11-07
期刊：
影响因子：
--
通讯作者：
Prather, Randall S
中科院分区：
文献类型：
--
作者：
Lai, Liangxue;Prather, Randall S
核移植（NT）是一个程序，通过它可以创建基因相同的个体。猪体细胞核移植技术包括卵母细胞的体外成熟、供体细胞的分离和处理、重构卵的人工激活、胚胎培养和胚胎移植等，近年来得到了广泛的研究，并在许多实验室成功地培育出了克隆猪。它为生产转基因猪提供了一种有效的方法，更重要的是，它是生产基因靶向猪的唯一途径。迄今为止，猪克隆技术已成功地用于生产表达绿色荧光蛋白的转基因猪，扩大转基因猪群，以及创建α-1，3-半乳糖基转移酶基因缺陷型猪。NT转基因猪的生产正处于从研究到实际应用的过渡阶段。虽然体细胞NT在猪中的效率，当作为所使用的卵母细胞的比例来测量发育到术语时，并不高，但是可以预期的是，对猪基因组进行特异性修饰的能力将导致该技术不仅对医学而且对农业产生巨大影响。
Nuclear transfer (NT) is a procedure by which genetically identical individuals can be created. The technology of pig somatic NT, including in vitro maturation of oocytes, isolation and treatment of donor cells, artificial activation of reconstructed oocytes, embryo culture and embryo transfer, has been intensively studied in recent years, resulting in birth of cloned pigs in many labs. While it provides an efficient method for producing transgenic pigs, more importantly, it is the only way to produce gene-targeted pigs. So far pig cloning has been successfully used to produce transgenic pigs expressing the green fluorescence protein, expand transgenic pig groups and create gene targeted pigs which are deficient of alpha-1,3-galactosyltransferase. The production of pigs with genetic modification by NT is now in the transition from investigation to practical use. Although the efficiency of somatic cell NT in pig, when measured as development to term as a proportion of oocytes used, is not high, it is anticipated that the ability of making specific modifications to the swine genome will result in this technology having a large impact not only on medicine but also on agriculture.