Genetic differences in Pneumocystis isolates recovered from immunocompetent infants and from adults with AIDS:: Epidemiological implications
Genetic differences in Pneumocystis isolates recovered from immunocompetent infants and from adults with AIDS:: Epidemiological implications
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DOI：
10.1086/497381
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发表时间：
2005-11-15
影响因子：
6.4
通讯作者：
Colley, DG
中科院分区：
文献类型：
--
作者：
Beard, CB;Fox, MR;Colley, DG
使用聚合酶链式反应分析、直接 DNA 测序和组织学染色来确定尸检时从 58 名不患有明显免疫缺陷的婴儿身上获取的肺组织标本中是否存在耶氏肺孢子虫。将这些标本的基因分型结果与 384 例人类免疫缺陷病毒 (HIV) 感染的肺孢子虫肺炎成人标本的基因分型结果进行了比较。在从婴儿获得的样本中，线粒体大亚基 rRNA 和二氢蝶酸合酶基因座中分别检测到 P. jirovecii DNA，比例分别为 100% 和 53%。所有从成虫身上获得的样本在两个位点的 P. jirovecii 检测结果均呈阳性。两个群体中两个基因座的基因型分布显着不同 (P < .001)。对免疫功能正常的婴儿和感染艾滋病毒的成人中传播的不同毒株的观察表明，独立的传播周期值得进一步研究。
Polymerase chain reaction analysis, direct DNA sequencing, and histological staining were used to determine whether Pneumocystis jirovecii was present in lung tissue specimens obtained, at autopsy, from 58 infants without identifiable immunodeficiency. The results of genotyping of these specimens were compared with the results of genotyping of specimens obtained from 384 human immunodeficiency virus (HIV)-infected adults with Pneumocystis pneumonia. P. jirovecii DNA was detected at the mitochondrial large subunit rRNA and dihydropteroate synthase loci in 100% and 53%, respectively, of the specimens obtained from infants. All specimens obtained from adults tested positive for P. jirovecii at both loci. Genotype distributions at both loci were significantly different in the 2 populations (P < .001). The observation of different strains circulating in immunocompetent infants and HIV-infected adults suggests independent transmission cycles that warrant further study.