Variation in the expression of a transmembrane protein influences cell growth in Arabidopsis thaliana petals by altering auxin availability
Variation in the expression of a transmembrane protein influences cell growth in Arabidopsis thaliana petals by altering auxin availability
复制标题
跨膜蛋白表达的变化通过改变生长素的可用性影响拟南芥花瓣的细胞生长
DOI：
10.1101/2020.01.05.895078
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Miller C
中科院分区：
文献类型：
--
作者：
Miller C
背景同一物种的植物可以表现出由基因决定的高度不同的器官大小和形状。定义这种形态多样性背后的遗传变异是进化研究的一个重要目标，它也有助于确定影响植物生长和发育的基因的功能。对诱变拟南芥种群的广泛筛选已经鉴定出影响器官大小和形状的多个基因和机制，但相对较少的研究利用自然种群的丰富多样性来鉴定参与生长控制的基因。结果我们筛选了相对良好表征的拟南芥生态型集合，以了解花瓣大小的变化。关联分析确定了 4 号染色体上的序列和基因表达变异，这些变异对花瓣面积的差异做出了重大贡献。 At4g16850（称为 KSK）编码一种假设的蛋白质，其表达的变化通过影响细胞大小而对器官大小的变化发挥着重要作用。 KSK 的过度表达导致花瓣更大，细胞更大，并促进雄蕊特征的形成。已知限制细胞生长的生长素反应基因的表达因 KSK 过度表达而减少。 KSK 过表达品系中的 ANT 表达也有所减少，这与花特性的改变一致。 KSK 过表达细胞中生长素的可用性降低，与生长素响应基因表达的变化一致。因此，KSK 可能会影响花瓣发育过程中生长素的可用性。结论了解遗传变异如何影响植物生长对于进化和机制研究都很重要。我们使用拟南芥天然群体来鉴定拟南芥生态型启动子区域的序列变异，这些变异介导了先前未表征的膜蛋白的表达差异。这种变化导致花瓣生长过程中生长素可用性和细胞大小的改变。
BackgroundThe same species of plant can exhibit highly diverse sizes and shapes of organs that are genetically determined. Defining genetic variation underlying this morphological diversity is an important objective in evolutionary studies and it also helps identify the functions of genes influencing plant growth and development. Extensive screens of mutagenised Arabidopsis populations have identified multiple genes and mechanisms affecting organ size and shape, but relatively few studies have exploited the rich diversity of natural populations to identify genes involved in growth control.ResultsWe screened a relatively well characterised collection of Arabidopsis thaliana ecotypes for variation in petal size. Association analyses identified sequence and gene expression variation on chromosome 4 that made a substantial contribution to differences in petal area. Variation in expression of At4g16850 (named as KSK), encoding a hypothetical protein, had a substantial role on variation in organ size by influencing cell size. Over-expression of KSK led to larger petals with larger cells and promoted the formation of stamenoid features. The expression of auxin-responsive genes known to limit cell growth was reduced in response to KSK over-expression. ANT expression was also reduced in KSK over-expression lines, consistent with altered floral identities. Auxin availability was reduced in KSK over-expressing cells, consistent with changes in auxin-responsive gene expression. KSK may therefore influence auxin availability during petal development.ConclusionsUnderstanding how genetic variation influences plant growth is important for both evolutionary and mechanistic studies. We used natural populations of Arabidopsis thaliana to identify sequence variation in a promoter region of Arabidopsis ecotypes that mediated differences in the expression of a previously uncharacterised membrane protein. This variation contributed to altered auxin availability and cell size during petal growth.
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