Expression of saliva-binding epitopes of the Porphyromonas gingivalis FimA protein on the surface of Streptococcus gordonii.
Expression of saliva-binding epitopes of the Porphyromonas gingivalis FimA protein on the surface of Streptococcus gordonii.
复制标题
牙龈卟啉单胞菌 FimA 蛋白唾液结合表位在戈登链球菌表面的表达。
DOI：
10.1006/bbrc.1999.0616
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Genco,RJ
中科院分区：
文献类型：
--
作者：
Sharma,A;Honma,K;Sojar,HT;Hruby,DE;Kuramitsu,HK;Genco,RJ
牙龈卟啉单胞菌是一种革兰氏阴性口腔厌氧细菌，与牙周炎的发病和发展有关。就会。介导细菌粘附宿主口腔部位并诱导宿主炎症反应的牙龈菌原被认为是一种潜在的抗原候选物。用于疫苗开发。本研究旨在产生表达p的主要亚基蛋白（FimA）的哥氏链球菌载体。作为潜在的牙周炎活疫苗的牙龈菌膜试验。我们在此报道了p的c端唾液结合表位的表达。牙龈表面的fima。gordoniand证明含有游离半胱氨酸残基的结构域在s表面表达不良。gordonii。
Porphyromonas gingivalis,a gram-negative oral anaerobic bacterium, has been implicated in the onset and development of periodontitis. TheP. gingivalisfimbriae which mediate bacterial adherence to host oral sites and induce host inflammatory responses have been suggested as a potential antigen candidate. for vaccine development. This study was undertaken to generateStreptococcus gordoniivectors expressing the major subunit protein (FimA) ofP. gingivalisfimbriae for testing as a potential live vaccine against periodontitis. We report here the expression of the C-terminal saliva-binding epitopes ofP. gingivalisFimA on the surface ofS. gordoniiand demonstrate that domains containing free cysteine residues are poorly expressed on the surface ofS. gordonii.