MULTIPLE FATTY-ACID BINDING TO ALBUMIN IN HUMAN BLOOD-PLASMA
MULTIPLE FATTY-ACID BINDING TO ALBUMIN IN HUMAN BLOOD-PLASMA
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DOI：
10.1111/j.1432-1033.1990.tb15495.x
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发表时间：
1990-04-30
期刊：
影响因子：
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通讯作者：
PEDERSEN, AO
中科院分区：
文献类型：
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作者：
BRODERSEN, R;ANDERSEN, S;PEDERSEN, AO
用透析交换率技术研究了长链脂肪酸在血清或血浆中与人血清白蛋白的结合平衡。将棕榈酸酯添加到体外柠檬酸血浆中，观察到有6到10个棕榈酸酯分子以几乎相等的亲和力与白蛋白结合。在体内的观察得出了以下一系列类似的结果：(a)在禁食、运动和冷水澡后脂肪酸浓度增加的两名志愿者中：(b)通过静脉注射卵磷脂/糖胆酸混合胶束制剂，3名男性志愿者体内非酯化脂肪酸浓度高达4.6 mM; (c) 81名糖尿病患者。血清或血浆中棕榈酸盐的结合模式与将棕榈酸盐加入纯白蛋白缓冲溶液中观察到的结合模式本质上不同，当两个分子紧密结合时，另外大约有四个分子的亲和力较低。这种差异部分可以解释为血浆中氯离子的存在，降低了前两种脂肪酸分子结合的亲和力，部分可以解释为血浆中几种混合脂肪酸分子的结合更容易。
Binding equilibria of long-chain fatty acids to human serum albumin, in serum or plasma, were studied by a dialysis exchange rate technique. Palmitate was added to citrated plasma in vitro and it was observed that between six and ten palmitate molecules were bound to albumin with nearly equal affinity. Observations in vivo gave similar results in the following series: (a) in two volunteers with increased fatty acid concentrations after fasting, exercise, and a cold shower: (b) in three male volunteers in whom high concentrations of non-esterified fatty acids, up to 4.6 mM, were induced by intravenous administration of a preparation of lecithin/glycocholate mixed micelles, and (c) in 81 patients with diabetes mellitus, type I. The binding pattern of palmitate in serum or plasma is essentially different from that observed with palmitate added to buffered solutions of pure albumin when two molecules are tightly bound and about four additional molecules with lower affinity. The differences may partly be explained by the presence of chloride ions in blood plasma, reducing the affinity for binding of the first two fatty acid molecules, and partly by facilitated binding of several molecules of mixed fatty acids, as found in plasma.