Metabolic Profiles Reveal Changes in Wild and Cultivated Soybean Seedling Leaves under Salt Stress.
Metabolic Profiles Reveal Changes in Wild and Cultivated Soybean Seedling Leaves under Salt Stress.
复制标题
DOI：
10.1371/journal.pone.0159622
复制
发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Shi L
中科院分区：
文献类型：
--
作者：
Zhang J;Yang D;Li M;Shi L
阐明植物盐胁迫反应的代谢机制将有助于进一步优化盐碱地作物高产育种和栽培。本文采用气相色谱-质谱（GC-MS）代谢组学技术，对栽培大豆（Glycine max）和野生大豆（Glycine soja）在中性盐和碱盐胁迫下的68种差异代谢产物进行了分析，旨在揭示栽培大豆和野生大豆在耐盐性方面的生理和分子差异。两种盐胁迫下生长参数的比较表明，野生大豆的抑制程度低于栽培大豆，尤其是在碱盐胁迫下。中性盐胁迫下野生大豆中苯丙氨酸、天冬酰胺、柠康酸、柠苹果酸、柠檬酸和α-酮基谷氨酸含量显著高于栽培大豆;碱盐胁迫下野生大豆中棕榈酸、二十四烷酸、葡萄糖、柠檬酸和α-酮基谷氨酸含量显著高于栽培大豆。进一步的研究表明，野生大豆的耐盐能力主要是基于有机酸和氨基酸的合成，以及在中性盐胁迫下三羧酸循环的活跃。此外，代谢产物谱分析表明，β-氧化、糖酵解和柠檬酸循环在盐碱胁迫下发挥重要作用。本研究结果为进一步了解野生大豆的耐盐机制提供了理论依据，并为提高野生大豆的产量和培育耐盐大豆品种提供了重要参考。
Clarification of the metabolic mechanisms underlying salt stress responses in plants will allow further optimization of crop breeding and cultivation to obtain high yields in saline-alkali land. Here, we characterized 68 differential metabolites of cultivated soybean (Glycine max) and wild soybean (Glycine soja) under neutral-salt and alkali-salt stresses using gas chromatography-mass spectrometry (GC-MS)-based metabolomics, to reveal the physiological and molecular differences in salt tolerance. According to comparisons of growth parameters under the two kinds of salt stresses, the level of inhibition in wild soybean was lower than in cultivated soybean, especially under alkali-salt stress. Moreover, wild soybean contained significantly higher amounts of phenylalanine, asparagine, citraconic acid, citramalic acid, citric acid and α-ketoglutaric acid under neutral-salt stress, and higher amounts of palmitic acid, lignoceric acid, glucose, citric acid and α-ketoglutaric acid under alkali-salt stress, than cultivated soybean. Further investigations demonstrated that the ability of wild soybean to salt tolerance was mainly based on the synthesis of organic and amino acids, and the more active tricarboxylic acid cycle under neutral-salt stress. In addition, the metabolite profiling analysis suggested that the energy generation from β-oxidation, glycolysis and the citric acid cycle plays important roles under alkali-salt stress. Our results extend the understanding of mechanisms involved in wild soybean salt tolerance and provide an important reference for increasing yields and developing salt-tolerant soybean cultivars.